Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15253 and 15254 are separated by 1312 nucleotides15254 and 15255 are separated by 200 nucleotides15255 and 15256 are separated by 232 nucleotides15256 and 15257 are separated by 479 nucleotides mRNA_6885: 15253 - KOG0565 Inositol polyphosphate 5-phosphatase and related proteins, at 351,614 to 354,932 15253 mRNA_6886: 15254 - K09493 CCT1, TCP1 T-complex protein 1 subunit alpha, at 356,245 to 358,443 15254 mRNA_6887: 15255 - KOG2793 Putative N2,N2-dimethylguanosine tRNA methyltransferase, at 358,644 to 359,526 15255 mRNA_6888: 15256 - K04422 MAP3K13, LZK mitogen-activated protein kinase kinase kinase 13, at 359,759 to 363,523 15256 mRNA_6889: 15257 - K17065 DNM1L dynamin 1-like protein, at 364,003 to 366,716 15257
Group Condition mRNA_6885 mRNA_6886 mRNA_6887 mRNA_6888 mRNA_6889
15253 15254 15255 15256 15257
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.0 -1.6 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 N.D. -0.3 -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 N.D. +0.4 -1.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 N.D. +0.2 -0.8 N.D.
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.5 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.4 -0.8 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. +0.2 -0.4 N.D.
growth on oleic acid YNB Glucose +0.2 N.D. +0.2 -0.8 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. +0.2 -0.3 N.D.
auxotrophy YNB Glucose +0.2 N.D. -0.4 +0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. -0.2 +0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.0 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. -0.3 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.3 +0.0 N.D.
growth on ypd YPD -0.0 N.D. +0.2 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.0 +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. -0.0 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.0 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.0 +0.3 N.D.
auxotrophy Dropout Complete +0.1 N.D. +0.1 +0.4 N.D.
growth on oleic acid YNB Oleic Acid +0.3 N.D. +0.5 +0.1 N.D.
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