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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
15254 and 15255 are separated by 200 nucleotides
15255 and 15256 are separated by 232 nucleotides
15256 and 15257 are separated by 479 nucleotides
15257 and 15258 are separated by 702 nucleotides
mRNA_6886: 15254 - K09493 CCT1, TCP1 T-complex protein 1 subunit alpha, at 356,245 to 358,443
15254
mRNA_6887: 15255 - KOG2793 Putative N2,N2-dimethylguanosine tRNA methyltransferase, at 358,644 to 359,526
15255
mRNA_6888: 15256 - K04422 MAP3K13, LZK mitogen-activated protein kinase kinase kinase 13, at 359,759 to 363,523
15256
mRNA_6889: 15257 - K17065 DNM1L dynamin 1-like protein, at 364,003 to 366,716
15257
mRNA_6890: 15258 - K09496 CCT4 T-complex protein 1 subunit delta, at 367,419 to 369,510
15258
Group
Condition
mRNA
_6886
mRNA
_6887
mRNA
_6888
mRNA
_6889
mRNA
_6890
15254
15255
15256
15257
15258
facs on lipid accumulation
Sorted No BODIPY Gate
N.D.
+0.0
-1.6
N.D.
N.D.
growth on ricinoleic acid
YNB Glucose
N.D.
-0.5
-0.3
N.D.
N.D.
growth on ricinoleic acid
YNB Ricinoleic Acid
N.D.
-0.3
-0.3
N.D.
N.D.
facs on lipid accumulation
Sorted Low BODIPY Gate
N.D.
+0.4
-1.1
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
N.D.
+0.2
-0.8
N.D.
N.D.
growth on oleic acid
YNB Glucose
N.D.
+0.2
-0.8
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Low Buoyancy
N.D.
+0.4
-0.8
N.D.
N.D.
auxotrophy
YNB Glucose
N.D.
-0.4
+0.0
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Methionine
N.D.
-0.3
+0.0
N.D.
N.D.
ypd buoyancy
40 hrs High Buoyancy
N.D.
-0.2
+0.0
N.D.
N.D.
facs on lipid accumulation
Sorted High BODIPY Gate
N.D.
+0.2
-0.3
N.D.
N.D.
growth on ricinoleic acid
YNB Methylricinoleic Acid
N.D.
+0.0
-0.2
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs Median Buoyancy
N.D.
+0.2
-0.4
N.D.
N.D.
growth on ypd
YPD
N.D.
+0.2
-0.1
N.D.
N.D.
ypd buoyancy
40 hrs Median Buoyancy
N.D.
-0.0
+0.2
N.D.
N.D.
ypd buoyancy
40 hrs Low Buoyancy
N.D.
+0.0
+0.2
N.D.
N.D.
auxotrophy
YNB Glucose 75mM Arginine
N.D.
+0.0
+0.2
N.D.
N.D.
ypd buoyancy
40 hrs No Buoyancy Separation
N.D.
-0.3
+0.6
N.D.
N.D.
buoyancy on lipid accumulation
40 hrs High Buoyancy
N.D.
-0.0
+0.3
N.D.
N.D.
auxotrophy
Dropout Complete
N.D.
+0.1
+0.4
N.D.
N.D.
growth on oleic acid
YNB Oleic Acid
N.D.
+0.5
+0.1
N.D.
N.D.
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