Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15261 and 15262 are separated by 1663 nucleotides15262 and 15263 are separated by 247 nucleotides15263 and 15264 are separated by 1353 nucleotides15264 and 15265 are separated by 620 nucleotides mRNA_6893: 15261 - Hypothetical Protein, at 372,844 to 373,603 15261 mRNA_6894: 15262 - K14326 UPF1, RENT1 regulator of nonsense transcripts 1, at 375,267 to 378,241 15262 mRNA_6895: 15263 - K18183 COX19 cytochrome c oxidase assembly protein subunit 19, at 378,489 to 378,951 15263 mRNA_6896: 15264 - KOG1399 Flavin-containing monooxygenase, at 380,305 to 381,908 15264 mRNA_6897: 15265 - Hypothetical Protein, at 382,529 to 383,336 15265
Group Condition mRNA_6893 mRNA_6894 mRNA_6895 mRNA_6896 mRNA_6897
15261 15262 15263 15264 15265
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 N.D. N.D. +0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. N.D. -0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. -0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. N.D. +0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. N.D. -0.1 -0.2
auxotrophy YNB Glucose -0.3 N.D. N.D. +0.1 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.3 N.D. N.D. +0.2 +0.2
auxotrophy Dropout Complete +0.1 N.D. N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. N.D. +0.5 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. N.D. +0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 N.D. N.D. +0.1 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 N.D. N.D. +0.2 +0.1
growth on ricinoleic acid YNB Glucose +0.3 N.D. N.D. +0.5 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. +0.0 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 N.D. N.D. +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.7 N.D. N.D. +0.2 -0.3
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. +0.4 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 N.D. N.D. -0.2 +0.2
growth on oleic acid YNB Glucose +0.5 N.D. N.D. +0.4 +0.2
growth on ypd YPD -0.2 N.D. N.D. +0.8 +0.6
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