Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15262 and 15263 are separated by 247 nucleotides15263 and 15264 are separated by 1353 nucleotides15264 and 15265 are separated by 620 nucleotides15265 and 15266 are separated by 410 nucleotides mRNA_6894: 15262 - K14326 UPF1, RENT1 regulator of nonsense transcripts 1, at 375,267 to 378,241 15262 mRNA_6895: 15263 - K18183 COX19 cytochrome c oxidase assembly protein subunit 19, at 378,489 to 378,951 15263 mRNA_6896: 15264 - KOG1399 Flavin-containing monooxygenase, at 380,305 to 381,908 15264 mRNA_6897: 15265 - Hypothetical Protein, at 382,529 to 383,336 15265 mRNA_6898: 15266 - HMMPfam-Glutathione S-transferase, N-terminal domain-PF13417,SUPERFAMILY--SSF52833, at 383,747 to 384,768 15266
Group Condition mRNA_6894 mRNA_6895 mRNA_6896 mRNA_6897 mRNA_6898
15262 15263 15264 15265 15266
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.1 -0.2 -0.4
auxotrophy Dropout Complete N.D. N.D. -0.1 +0.2 -0.6
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.1 -0.5 -0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.1 -0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 -0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 +0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 -0.3 +0.0
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.5 -0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.0 -0.2 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.1 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.4 +0.0 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.1 -0.0 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 -0.2 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.2 +0.2 -0.1
auxotrophy YNB Glucose N.D. N.D. +0.1 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.5 -0.4 +0.2
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 +0.4 +0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.4 +0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.2 +0.2 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.2 +0.1 +0.4
growth on ypd YPD N.D. N.D. +0.8 +0.6 -0.2
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