Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15273 and 15274 are separated by 346 nucleotides15274 and 15275 are separated by 159 nucleotides15275 and 15276 are separated by 570 nucleotides15276 and 15277 are separated by 231 nucleotides mRNA_6905: 15273 - K14300 NUP133 nuclear pore complex protein Nup133, at 397,122 to 402,305 15273 mRNA_6906: 15274 - Hypothetical Protein, at 402,652 to 403,078 15274 mRNA_6907: 15275 - K14016 UFD1 ubiquitin fusion degradation protein 1, at 403,238 to 404,908 15275 mRNA_6908: 15276 - K01907 AACS, acsA acetoacetyl-CoA synthetase, at 405,479 to 408,297 15276 mRNA_6909: 15277 - K14315 NDC1, TMEM48 nucleoporin NDC1, at 408,529 to 411,391 15277
Group Condition mRNA_6905 mRNA_6906 mRNA_6907 mRNA_6908 mRNA_6909
15273 15274 15275 15276 15277
facs on lipid accumulation Sorted No BODIPY Gate -2.0 N.D. N.D. +0.2 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -1.9 N.D. N.D. +0.0 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 N.D. N.D. -0.3 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -2.1 N.D. N.D. +0.3 +0.1
growth on ricinoleic acid YNB Glucose -0.9 N.D. N.D. +0.1 -0.8
auxotrophy YNB Glucose 75mM Methionine -0.8 N.D. N.D. -0.2 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.9 N.D. N.D. -0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. N.D. -0.2 -0.1
auxotrophy YNB Glucose -0.5 N.D. N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.2 N.D. N.D. -0.0 +0.3
auxotrophy Dropout Complete -0.6 N.D. N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 N.D. N.D. -0.1 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 N.D. N.D. -0.5 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. N.D. +0.0 -0.2
growth on oleic acid YNB Glucose -0.7 N.D. N.D. +0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. N.D. -0.0 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.4 N.D. N.D. -0.2 -0.5
growth on oleic acid YNB Oleic Acid +0.3 N.D. N.D. -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +1.0 N.D. N.D. -0.2 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.9 N.D. N.D. -0.2 +0.2
growth on ypd YPD +1.6 N.D. N.D. -0.3 +0.2
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