Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15276 and 15277 are separated by 231 nucleotides15277 and 15278 are separated by 337 nucleotides15278 and 15279 are separated by 660 nucleotides15279 and 15280 are separated by 237 nucleotides mRNA_6908: 15276 - K01907 AACS, acsA acetoacetyl-CoA synthetase, at 405,479 to 408,297 15276 mRNA_6909: 15277 - K14315 NDC1, TMEM48 nucleoporin NDC1, at 408,529 to 411,391 15277 mRNA_6910: 15278 - KOG2287 Galactosyltransferases, at 411,729 to 414,888 15278 mRNA_6911: 15279 - K03660 OGG1 N-glycosylase/DNA lyase, at 415,549 to 417,203 15279 mRNA_6912: 15280 - K12833 SF3B14 pre-mRNA branch site protein p14, at 417,441 to 418,066 15280
Group Condition mRNA_6908 mRNA_6909 mRNA_6910 mRNA_6911 mRNA_6912
15276 15277 15278 15279 15280
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.5 N.D. -0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.8 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.4 N.D. +0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.5 N.D. +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.3 N.D. -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.0 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.3 N.D. +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 -0.1 N.D. +0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.2 N.D. -0.0 N.D.
auxotrophy YNB Glucose -0.1 -0.3 N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.2 N.D. +0.1 N.D.
growth on oleic acid YNB Oleic Acid -0.0 +0.1 N.D. -0.2 N.D.
growth on ypd YPD -0.3 +0.2 N.D. -0.1 N.D.
auxotrophy Dropout Complete -0.1 -0.2 N.D. +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.3 N.D. +0.1 N.D.
growth on oleic acid YNB Glucose +0.2 +0.0 N.D. -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 +0.3 N.D. -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.1 N.D. -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.0 +0.0 N.D. +0.3 N.D.
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