Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15278 and 15279 are separated by 660 nucleotides15279 and 15280 are separated by 237 nucleotides15280 and 15281 are separated by 1494 nucleotides15281 and 15282 are separated by 715 nucleotides mRNA_6910: 15278 - KOG2287 Galactosyltransferases, at 411,729 to 414,888 15278 mRNA_6911: 15279 - K03660 OGG1 N-glycosylase/DNA lyase, at 415,549 to 417,203 15279 mRNA_6912: 15280 - K12833 SF3B14 pre-mRNA branch site protein p14, at 417,441 to 418,066 15280 mRNA_6913: 15281 - Hypothetical Protein, at 419,561 to 420,635 15281 mRNA_6914: 15282 - KOG0254 Predicted transporter (major facilitator superfamily), at 421,351 to 423,576 15282
Group Condition mRNA_6910 mRNA_6911 mRNA_6912 mRNA_6913 mRNA_6914
15278 15279 15280 15281 15282
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.2 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. -0.1 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.2 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 N.D. +0.1 -0.4
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. +0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.2 N.D. +0.4 +0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. -0.0 N.D. +0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.0 N.D. +0.2 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 N.D. -0.1 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.1 N.D. +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 N.D. +0.0 +0.2
growth on ypd YPD N.D. -0.1 N.D. +0.1 +0.3
growth on oleic acid YNB Glucose N.D. -0.2 N.D. +0.3 +0.2
growth on ricinoleic acid YNB Glucose N.D. +0.0 N.D. -0.0 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 N.D. +0.1 +0.4
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. +0.1 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 N.D. +0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 N.D. -0.1 +0.5
auxotrophy Dropout Complete N.D. +0.1 N.D. +0.2 +0.4
auxotrophy YNB Glucose N.D. +0.2 N.D. +0.3 +0.7
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