Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15283 and 15284 are separated by 300 nucleotides15284 and 15285 are separated by 236 nucleotides15285 and 15286 are separated by 1258 nucleotides15286 and 15287 are separated by 252 nucleotides mRNA_6915: 15283 - K01130 E3.1.6.1, aslA arylsulfatase, at 424,136 to 426,200 15283 mRNA_6916: 15284 - SUPERFAMILY--SSF52833, at 426,501 to 427,574 15284 mRNA_6917: 15285 - K04883 KCNAB2 potassium voltage-gated channel Shaker-related subfamily A, beta member 2, at 427,811 to 428,744 15285 mRNA_6918: 15286 - HMMPfam-Coiled-coil domain-containing protein 56-PF09813, at 430,003 to 430,506 15286 mRNA_6919: 15287 - K07556 ATPeAF2, ATPAF2, ATP12 ATP synthase mitochondrial F1 complex assembly factor 2, at 430,759 to 431,959 15287
Group Condition mRNA_6915 mRNA_6916 mRNA_6917 mRNA_6918 mRNA_6919
15283 15284 15285 15286 15287
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 -0.5 +0.4 N.D. N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -0.2 -0.5 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 -0.3 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.0 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.8 +0.1 +0.5 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.4 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.4 +0.3 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.3 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.0 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 +0.3 +0.2 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.0 -0.1 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 +0.0 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 -0.3 +0.8 N.D. N.D.
growth on oleic acid YNB Glucose -0.4 +0.1 +0.6 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.3 +0.3 N.D. N.D.
growth on ypd YPD +0.1 -0.0 +0.4 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 +0.2 -0.0 N.D. N.D.
growth on oleic acid YNB Oleic Acid -0.4 +0.0 +1.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.1 +0.7 N.D. N.D.
auxotrophy Dropout Complete -0.2 -0.1 +1.0 N.D. N.D.
auxotrophy YNB Glucose -0.1 -0.2 +1.2 N.D. N.D.
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