Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15290 and 15291 are separated by 872 nucleotides15291 and 15292 are separated by 553 nucleotides15292 and 15293 are separated by 521 nucleotides15293 and 15294 are separated by 176 nucleotides mRNA_6922: 15290 - KOG4121 Nuclear pore complex, Nup133 component (sc Nup133), at 436,165 to 438,557 15290 mRNA_6923: 15291 - Hypothetical Protein, at 439,430 to 443,984 15291 mRNA_6924: 15292 - BLAST PC-Esterase [Xanthophyllomyces dendrorhous], at 444,538 to 446,309 15292 mRNA_6925: 15293 - K02331 POL5, MYBBP1A DNA polymerase phi, at 446,831 to 450,905 15293 mRNA_6926: 15294 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 451,082 to 452,962 15294
Group Condition mRNA_6922 mRNA_6923 mRNA_6924 mRNA_6925 mRNA_6926
15290 15291 15292 15293 15294
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.4 -0.1 N.D. -2.0
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.3 -0.2 N.D. -1.5
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.4 +0.2 N.D. -1.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 +0.0 N.D. -1.1
auxotrophy Dropout Complete -0.4 -0.1 +0.4 N.D. -1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.0 +0.0 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.1 -0.0 N.D. -1.0
growth on ricinoleic acid YNB Glucose -0.3 -0.2 -0.1 N.D. -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.4 -0.1 -0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.1 +0.1 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.2 +0.2 N.D. -0.4
growth on ypd YPD +0.4 +0.0 -0.1 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.0 +0.2 -0.2 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.1 +0.3 N.D. -0.0
auxotrophy YNB Glucose +0.2 +0.0 +0.5 N.D. -0.4
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.3 +0.0 N.D. -0.2
growth on oleic acid YNB Glucose -0.2 -0.1 -0.3 N.D. +1.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.1 -0.4 +0.6 N.D. +0.7
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.3 +0.3 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.1 +0.4 N.D. +0.8
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.3 +0.3 N.D. +0.5
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