Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15299 and 15300 are separated by 200 nucleotides15300 and 15301 are separated by 1000 nucleotides15301 and 15302 are separated by 180 nucleotides15302 and 15303 are separated by 111 nucleotides mRNA_6931: 15299 - KOG4844 Mitochondrial ribosomal protein S27, at 463,074 to 463,703 15299 mRNA_6932: 15300 - K00764 purF, PPAT amidophosphoribosyltransferase, at 463,904 to 466,074 15300 mRNA_6933: 15301 - KOG0810 SNARE protein Syntaxin 1 and related proteins, at 467,075 to 468,286 15301 mRNA_6934: 15302 - K12871 CCDC12 coiled-coil domain-containing protein 12, at 468,467 to 469,312 15302 mRNA_6935: 15303 - K12830 SF3B3, SAP130, RSE1 splicing factor 3B subunit 3, at 469,424 to 473,834 15303
Group Condition mRNA_6931 mRNA_6932 mRNA_6933 mRNA_6934 mRNA_6935
15299 15300 15301 15302 15303
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.0 -1.6 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.5 -0.7 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.2 -0.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.3 -0.8 N.D.
growth on oleic acid YNB Glucose N.D. N.D. -0.3 -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.7 +0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.6 -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.4 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.6 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.0 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.4 +0.3 N.D.
auxotrophy Dropout Complete N.D. N.D. +0.0 -0.0 N.D.
growth on ypd YPD N.D. N.D. -0.6 +0.7 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.0 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.4 -0.2 N.D.
auxotrophy YNB Glucose N.D. N.D. +0.0 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.6 +0.9 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.3 +0.6 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.0 +0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.3 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 +0.4 N.D.
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