Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15301 and 15302 are separated by 180 nucleotides15302 and 15303 are separated by 111 nucleotides15303 and 15304 are separated by 98 nucleotides15304 and 15305 are separated by 585 nucleotides mRNA_6933: 15301 - KOG0810 SNARE protein Syntaxin 1 and related proteins, at 467,075 to 468,286 15301 mRNA_6934: 15302 - K12871 CCDC12 coiled-coil domain-containing protein 12, at 468,467 to 469,312 15302 mRNA_6935: 15303 - K12830 SF3B3, SAP130, RSE1 splicing factor 3B subunit 3, at 469,424 to 473,834 15303 mRNA_6936: 15304 - K09669 FUT10 alpha-1,3-fucosyltransferase 10, at 473,933 to 475,521 15304 mRNA_6937: 15305 - K03781 katE, CAT, catB, srpA catalase, at 476,107 to 478,851 15305
Group Condition mRNA_6933 mRNA_6934 mRNA_6935 mRNA_6936 mRNA_6937
15301 15302 15303 15304 15305
growth on oleic acid YNB Oleic Acid -0.5 -0.7 N.D. -0.1 -0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -1.6 N.D. +0.3 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.8 N.D. -0.5 -0.2
growth on ricinoleic acid YNB Glucose -0.4 +0.3 N.D. -0.5 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.1 N.D. -0.4 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.7 N.D. +0.1 +0.2
growth on oleic acid YNB Glucose -0.3 -0.1 N.D. -0.2 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.6 +0.9 N.D. -0.6 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 +0.3 N.D. -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.6 N.D. -0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.4 N.D. -0.5 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 -1.0 N.D. +0.3 +0.2
auxotrophy Dropout Complete +0.0 -0.0 N.D. +0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.0 N.D. +0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 +0.3 N.D. +0.6 +0.1
auxotrophy YNB Glucose +0.0 +0.2 N.D. +0.3 -0.2
growth on ypd YPD -0.6 +0.7 N.D. +0.5 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 N.D. +0.4 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.5 N.D. +0.3 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 N.D. +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.4 -0.2 N.D. +0.3 +0.3
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