Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15302 and 15303 are separated by 111 nucleotides15303 and 15304 are separated by 98 nucleotides15304 and 15305 are separated by 585 nucleotides15305 and 15306 are separated by 113 nucleotides mRNA_6934: 15302 - K12871 CCDC12 coiled-coil domain-containing protein 12, at 468,467 to 469,312 15302 mRNA_6935: 15303 - K12830 SF3B3, SAP130, RSE1 splicing factor 3B subunit 3, at 469,424 to 473,834 15303 mRNA_6936: 15304 - K09669 FUT10 alpha-1,3-fucosyltransferase 10, at 473,933 to 475,521 15304 mRNA_6937: 15305 - K03781 katE, CAT, catB, srpA catalase, at 476,107 to 478,851 15305 mRNA_6938: 15306 - SUPERFAMILY--SSF56801, at 478,965 to 480,801 15306
Group Condition mRNA_6934 mRNA_6935 mRNA_6936 mRNA_6937 mRNA_6938
15302 15303 15304 15305 15306
facs on lipid accumulation Sorted High BODIPY Gate -1.6 N.D. +0.3 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. -0.4 +0.1 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.8 N.D. -0.5 -0.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -1.0 N.D. +0.3 +0.2 -0.5
growth on oleic acid YNB Oleic Acid -0.7 N.D. -0.1 -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. -0.5 +0.1 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 N.D. -0.5 -0.2 -0.3
growth on ricinoleic acid YNB Glucose +0.3 N.D. -0.5 -0.2 +0.0
growth on oleic acid YNB Glucose -0.1 N.D. -0.2 -0.0 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.7 N.D. +0.1 +0.2 +0.3
auxotrophy Dropout Complete -0.0 N.D. +0.2 -0.0 -0.2
growth on ypd YPD +0.7 N.D. +0.5 -0.1 -1.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. -0.3 +0.3 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.9 N.D. -0.6 +0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.2 N.D. +0.3 +0.3 -0.3
auxotrophy YNB Glucose 75mM Methionine +0.5 N.D. +0.3 -0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.2 +0.1 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.4 -0.0 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. +0.6 +0.1 -0.5
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.1 +0.1 +0.2
auxotrophy YNB Glucose +0.2 N.D. +0.3 -0.2 +0.3
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