Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15307 and 15308 are separated by 68 nucleotides15308 and 15309 are separated by 346 nucleotides15309 and 15310 are separated by 127 nucleotides15310 and 15311 are separated by 187 nucleotides mRNA_6939: 15307 - K17973 NAA25, MDM20 N-terminal acetyltransferase B complex non-catalytic subunit, at 480,977 to 484,798 15307 mRNA_6940: 15308 - KOG2816 Predicted transporter ADD1 (major facilitator superfamily), at 484,867 to 487,020 15308 mRNA_6941: 15309 - K02154 ATPeV0A, ATP6N V-type H+-transporting ATPase subunit a, at 487,367 to 491,016 15309 mRNA_6942: 15310 - K15029 EIF3L translation initiation factor 3 subunit L, at 491,144 to 493,608 15310 mRNA_6943: 15311 - SUPERFAMILY--SSF52047, at 493,796 to 495,109 15311
Group Condition mRNA_6939 mRNA_6940 mRNA_6941 mRNA_6942 mRNA_6943
15307 15308 15309 15310 15311
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. +0.0 -1.7
auxotrophy YNB Glucose N.D. -0.3 N.D. -0.4 -0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 N.D. -0.8 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.4 N.D. -0.7 -0.0
growth on ricinoleic acid YNB Glucose N.D. +0.2 N.D. -1.3 +0.1
auxotrophy Dropout Complete N.D. -0.1 N.D. -0.5 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.3 N.D. -0.2 -0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 N.D. -0.7 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.1 N.D. -1.0 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.1 N.D. -0.6 +0.1
growth on oleic acid YNB Glucose N.D. +0.4 N.D. -0.9 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.3 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.4 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.3 -0.0
growth on ypd YPD N.D. +0.4 N.D. +0.0 -0.0
ypd buoyancy 40 hrs High Buoyancy N.D. +0.2 N.D. +0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 N.D. +0.5 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. +1.0 -0.0
growth on oleic acid YNB Oleic Acid N.D. -0.2 N.D. +1.3 +0.2
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