Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15317 and 15318 are separated by 1022 nucleotides15318 and 15319 are separated by 586 nucleotides15319 and 15320 are separated by 280 nucleotides15320 and 15321 are separated by 128 nucleotides mRNA_6949: 15317 - HMMPfam-Cenp-O kinetochore centromere component-PF09496, at 506,847 to 507,636 15317 mRNA_6950: 15318 - KOG4505 Na+/H+ antiporter, at 508,659 to 510,404 15318 mRNA_6951: 15319 - ProSiteProfiles-Ribonuclease III family domain profile.-PS50142,SUPERFAMILY--SSF69065, at 510,991 to 511,843 15319 mRNA_6952: 15320 - K11858 USP48 ubiquitin carboxyl-terminal hydrolase 48, at 512,124 to 517,290 15320 mRNA_6953: 15321 - K13719 OTU1, YOD1 ubiquitin thioesterase OTU1, at 517,419 to 518,782 15321
Group Condition mRNA_6949 mRNA_6950 mRNA_6951 mRNA_6952 mRNA_6953
15317 15318 15319 15320 15321
facs on lipid accumulation Sorted No BODIPY Gate -0.4 -3.8 -0.3 -0.6 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -3.5 -0.1 -1.2 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -2.9 -0.4 +0.0 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -1.0 +0.1 -0.0 +0.6
ypd buoyancy 40 hrs Low Buoyancy +0.0 -1.0 +0.6 -0.2 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.2 -0.6 -0.4 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 +0.6 -0.5 -0.9 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.7 +0.6 -0.1 -0.0
ypd buoyancy 40 hrs High Buoyancy -0.0 -0.0 +0.1 -0.0 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 +0.2 +0.7 -0.1 -0.1
growth on ricinoleic acid YNB Glucose +0.5 +0.4 -0.4 -0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.3 +0.5 -0.4 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.4 +0.0 +1.0 -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 +0.3 +0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.1 +0.7 -0.1 -0.0
growth on ypd YPD +0.6 +0.2 +0.0 +0.3 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.3 +0.8 -0.2 -0.1
growth on oleic acid YNB Glucose +0.1 +0.2 +0.7 -0.0 +0.1
auxotrophy Dropout Complete +0.2 +0.3 +0.8 +0.1 -0.3
auxotrophy YNB Glucose +0.4 +0.2 +0.4 +0.0 +0.3
growth on oleic acid YNB Oleic Acid +0.2 +0.6 +0.7 -0.1 +0.0
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