Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15318 and 15319 are separated by 586 nucleotides15319 and 15320 are separated by 280 nucleotides15320 and 15321 are separated by 128 nucleotides15321 and 15322 are separated by 410 nucleotides mRNA_6950: 15318 - KOG4505 Na+/H+ antiporter, at 508,659 to 510,404 15318 mRNA_6951: 15319 - ProSiteProfiles-Ribonuclease III family domain profile.-PS50142,SUPERFAMILY--SSF69065, at 510,991 to 511,843 15319 mRNA_6952: 15320 - K11858 USP48 ubiquitin carboxyl-terminal hydrolase 48, at 512,124 to 517,290 15320 mRNA_6953: 15321 - K13719 OTU1, YOD1 ubiquitin thioesterase OTU1, at 517,419 to 518,782 15321 mRNA_6954: 15322 - K10591 NEDD4, RSP5 E3 ubiquitin-protein ligase NEDD4, at 519,193 to 522,362 15322
Group Condition mRNA_6950 mRNA_6951 mRNA_6952 mRNA_6953 mRNA_6954
15318 15319 15320 15321 15322
facs on lipid accumulation Sorted No BODIPY Gate -3.8 -0.3 -0.6 -0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -3.5 -0.1 -1.2 +0.4 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -2.9 -0.4 +0.0 +0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 -0.5 -0.9 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.6 -0.4 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -1.0 +0.6 -0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 +0.6 -0.1 -0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.0 +0.1 -0.0 +0.6 N.D.
growth on ricinoleic acid YNB Glucose +0.4 -0.4 -0.2 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.1 -0.0 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.5 -0.4 -0.2 N.D.
growth on ypd YPD +0.2 +0.0 +0.3 -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.3 +0.1 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.7 -0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.8 -0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 +0.7 -0.1 -0.1 N.D.
growth on oleic acid YNB Glucose +0.2 +0.7 -0.0 +0.1 N.D.
auxotrophy Dropout Complete +0.3 +0.8 +0.1 -0.3 N.D.
auxotrophy YNB Glucose +0.2 +0.4 +0.0 +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 +1.0 -0.1 +0.0 N.D.
growth on oleic acid YNB Oleic Acid +0.6 +0.7 -0.1 +0.0 N.D.
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