Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15321 and 15322 are separated by 410 nucleotides15322 and 15323 are separated by 652 nucleotides15323 and 15324 are separated by 507 nucleotides15324 and 15325 are separated by 113 nucleotides mRNA_6953: 15321 - K13719 OTU1, YOD1 ubiquitin thioesterase OTU1, at 517,419 to 518,782 15321 mRNA_6954: 15322 - K10591 NEDD4, RSP5 E3 ubiquitin-protein ligase NEDD4, at 519,193 to 522,362 15322 mRNA_6955: 15323 - ProSiteProfiles-APSES-type HTH DNA-binding domain profile.-PS51299,SUPERFAMILY--SSF54616, at 523,015 to 527,499 15323 mRNA_6956: 15324 - K00720 UGCG ceramide glucosyltransferase, at 528,007 to 529,680 15324 mRNA_6957: 15325 - K13100 CWC22 pre-mRNA-splicing factor CWC22, at 529,794 to 532,653 15325
Group Condition mRNA_6953 mRNA_6954 mRNA_6955 mRNA_6956 mRNA_6957
15321 15322 15323 15324 15325
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.6 -1.7 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. -1.0 -1.7 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. -0.7 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. -0.7 -0.9 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.3 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. -0.3 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.4 N.D. -0.1 -0.9 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.0 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 N.D. +0.1 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. -0.2 -0.2 N.D.
growth on ypd YPD -0.1 N.D. -0.1 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. -0.2 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 N.D. -0.1 -0.4 N.D.
growth on oleic acid YNB Glucose +0.1 N.D. -0.2 -0.1 N.D.
auxotrophy Dropout Complete -0.3 N.D. -0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. +0.1 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. +0.1 +0.2 N.D.
auxotrophy YNB Glucose +0.3 N.D. -0.1 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.0 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.3 +1.0 N.D.
growth on oleic acid YNB Oleic Acid +0.0 N.D. +0.2 +0.6 N.D.
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