Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15324 and 15325 are separated by 113 nucleotides15325 and 15326 are separated by 1350 nucleotides15326 and 15327 are separated by 309 nucleotides15327 and 15328 are separated by 546 nucleotides mRNA_6956: 15324 - K00720 UGCG ceramide glucosyltransferase, at 528,007 to 529,680 15324 mRNA_6957: 15325 - K13100 CWC22 pre-mRNA-splicing factor CWC22, at 529,794 to 532,653 15325 mRNA_6958: 15326 - K09238 ACE2 metallothionein expression activator, at 534,004 to 536,795 15326 mRNA_6959: 15327 - K14805 DDX24, MAK5 ATP-dependent RNA helicase DDX24/MAK5, at 537,105 to 540,222 15327 mRNA_6960: 15328 - K01469 OPLAH, OXP1, oplAH 5-oxoprolinase (ATP-hydrolysing), at 540,769 to 543,741 15328
Group Condition mRNA_6956 mRNA_6957 mRNA_6958 mRNA_6959 mRNA_6960
15324 15325 15326 15327 15328
facs on lipid accumulation Sorted Low BODIPY Gate -1.7 N.D. -2.4 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate -1.7 N.D. -1.4 N.D. -0.0
ypd buoyancy 40 hrs High Buoyancy -0.7 N.D. -1.7 N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.9 N.D. -1.3 N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. -0.3 N.D. -0.3
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. -0.5 N.D. -0.1
growth on ypd YPD -0.3 N.D. +0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.0 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. +0.2 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. +0.8 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.4 N.D. +0.6 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.3 N.D. +0.1
growth on oleic acid YNB Glucose -0.1 N.D. +0.6 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. +1.0 N.D. -0.1
growth on oleic acid YNB Oleic Acid +0.6 N.D. -0.3 N.D. +0.3
auxotrophy Dropout Complete +0.4 N.D. +0.2 N.D. +0.1
auxotrophy YNB Glucose +0.1 N.D. +0.5 N.D. +0.2
growth on ricinoleic acid YNB Glucose +0.3 N.D. +1.2 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. +1.9 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +1.0 N.D. +1.8 N.D. +0.3
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