Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15326 and 15327 are separated by 309 nucleotides15327 and 15328 are separated by 546 nucleotides15328 and 15329 are separated by 162 nucleotides15329 and 15330 are separated by 49 nucleotides mRNA_6958: 15326 - K09238 ACE2 metallothionein expression activator, at 534,004 to 536,795 15326 mRNA_6959: 15327 - K14805 DDX24, MAK5 ATP-dependent RNA helicase DDX24/MAK5, at 537,105 to 540,222 15327 mRNA_6960: 15328 - K01469 OPLAH, OXP1, oplAH 5-oxoprolinase (ATP-hydrolysing), at 540,769 to 543,741 15328 mRNA_6961: 15329 - Hypothetical Protein, at 543,904 to 547,836 15329 mRNA_6962: 15330 - K03132 TAF7 transcription initiation factor TFIID subunit 7, at 547,886 to 549,459 15330
Group Condition mRNA_6958 mRNA_6959 mRNA_6960 mRNA_6961 mRNA_6962
15326 15327 15328 15329 15330
facs on lipid accumulation Sorted Low BODIPY Gate -2.4 N.D. -0.4 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -1.7 N.D. -0.2 +0.0 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 N.D. -0.4 +0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -1.4 N.D. -0.0 -0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. -0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. -0.3 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. -0.4 -0.2 N.D.
growth on oleic acid YNB Oleic Acid -0.3 N.D. +0.3 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. -0.1 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 N.D. +0.0 -0.0 N.D.
growth on ypd YPD +0.1 N.D. +0.0 +0.2 N.D.
auxotrophy Dropout Complete +0.2 N.D. +0.1 +0.0 N.D.
growth on oleic acid YNB Glucose +0.6 N.D. +0.0 -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. +0.1 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 N.D. -0.0 -0.0 N.D.
auxotrophy YNB Glucose +0.5 N.D. +0.2 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +1.2 N.D. -0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +1.0 N.D. -0.1 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.8 N.D. +0.2 -0.0 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.9 N.D. +0.1 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +1.8 N.D. +0.3 -0.0 N.D.
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