Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15327 and 15328 are separated by 546 nucleotides15328 and 15329 are separated by 162 nucleotides15329 and 15330 are separated by 49 nucleotides15330 and 15331 are separated by 382 nucleotides mRNA_6959: 15327 - K14805 DDX24, MAK5 ATP-dependent RNA helicase DDX24/MAK5, at 537,105 to 540,222 15327 mRNA_6960: 15328 - K01469 OPLAH, OXP1, oplAH 5-oxoprolinase (ATP-hydrolysing), at 540,769 to 543,741 15328 mRNA_6961: 15329 - Hypothetical Protein, at 543,904 to 547,836 15329 mRNA_6962: 15330 - K03132 TAF7 transcription initiation factor TFIID subunit 7, at 547,886 to 549,459 15330 mRNA_6963: 15331 - Hypothetical Protein, at 549,842 to 550,144 15331
Group Condition mRNA_6959 mRNA_6960 mRNA_6961 mRNA_6962 mRNA_6963
15327 15328 15329 15330 15331
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 -0.0 N.D. -1.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 -0.1 N.D. -0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 -0.0 N.D. -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose N.D. -0.2 -0.1 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 -0.0 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 -0.2 N.D. +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 +0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 -0.0 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 -0.1 N.D. +0.1
growth on oleic acid YNB Glucose N.D. +0.0 -0.1 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.3 -0.0 N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.0 -0.0 N.D. +0.3
growth on oleic acid YNB Oleic Acid N.D. +0.3 -0.1 N.D. +0.1
auxotrophy Dropout Complete N.D. +0.1 +0.0 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.0 N.D. +0.5
auxotrophy YNB Glucose N.D. +0.2 +0.1 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 -0.0 N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -0.0 N.D. +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.1 N.D. +0.3
growth on ypd YPD N.D. +0.0 +0.2 N.D. +0.8
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