Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15333 and 15334 are separated by 359 nucleotides15334 and 15335 are separated by 251 nucleotides15335 and 15336 are separated by 315 nucleotides15336 and 15337 are separated by 144 nucleotides mRNA_6965: 15333 - Hypothetical Protein, at 553,877 to 555,010 15333 mRNA_6966: 15334 - K02210 MCM7, CDC47 DNA replication licensing factor MCM7, at 555,370 to 558,100 15334 mRNA_6967: 15335 - K19995 SCAMP secretory carrier-associated membrane protein, at 558,352 to 560,106 15335 mRNA_6968: 15336 - K00737 MGAT3 beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, at 560,422 to 561,850 15336 mRNA_6969: 15337 - K17261 CAP1_2, SRV2 adenylyl cyclase-associated protein, at 561,995 to 564,059 15337
Group Condition mRNA_6965 mRNA_6966 mRNA_6967 mRNA_6968 mRNA_6969
15333 15334 15335 15336 15337
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.5 N.D. -0.2 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 N.D. +0.0 -0.5 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. -0.5 -0.2 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 N.D. -0.5 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. -0.7 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 N.D. -0.2 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 N.D. +0.2 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. +0.0 -0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.1 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 N.D. -0.6 -0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.3 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. -0.5 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.3 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 N.D. -0.0 +0.1 N.D.
auxotrophy Dropout Complete +0.6 N.D. -0.1 -0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.4 N.D. +0.0 -0.3 N.D.
auxotrophy YNB Glucose +0.5 N.D. -0.1 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.5 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.7 -0.1 N.D.
growth on ypd YPD +0.6 N.D. -0.5 +0.7 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.7 +0.1 N.D.
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