Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15334 and 15335 are separated by 251 nucleotides15335 and 15336 are separated by 315 nucleotides15336 and 15337 are separated by 144 nucleotides15337 and 15338 are separated by 2063 nucleotides mRNA_6966: 15334 - K02210 MCM7, CDC47 DNA replication licensing factor MCM7, at 555,370 to 558,100 15334 mRNA_6967: 15335 - K19995 SCAMP secretory carrier-associated membrane protein, at 558,352 to 560,106 15335 mRNA_6968: 15336 - K00737 MGAT3 beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, at 560,422 to 561,850 15336 mRNA_6969: 15337 - K17261 CAP1_2, SRV2 adenylyl cyclase-associated protein, at 561,995 to 564,059 15337 mRNA_6970: 15338 - Hypothetical Protein, at 566,123 to 566,867 15338
Group Condition mRNA_6966 mRNA_6967 mRNA_6968 mRNA_6969 mRNA_6970
15334 15335 15336 15337 15338
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.7 -0.2 N.D. -0.3
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 +0.1 N.D. -0.5
growth on oleic acid YNB Glucose N.D. -0.5 -0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 -0.4 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 +0.1 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Glucose N.D. -0.3 -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.0 -0.3 N.D. +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 +0.1 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.0 -0.3 N.D. +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.4 N.D. +0.5
auxotrophy Dropout Complete N.D. -0.1 -0.5 N.D. +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -0.6 -0.1 N.D. +0.7
auxotrophy YNB Glucose N.D. -0.1 -0.2 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 -0.3 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.0 -0.5 N.D. +0.6
growth on ypd YPD N.D. -0.5 +0.7 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.7 -0.1 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.5 -0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 -0.3 N.D. +0.7
ypd buoyancy 40 hrs High Buoyancy N.D. +0.7 +0.1 N.D. +0.1
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