Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15335 and 15336 are separated by 315 nucleotides15336 and 15337 are separated by 144 nucleotides15337 and 15338 are separated by 2063 nucleotides15338 and 15339 are separated by 374 nucleotides mRNA_6967: 15335 - K19995 SCAMP secretory carrier-associated membrane protein, at 558,352 to 560,106 15335 mRNA_6968: 15336 - K00737 MGAT3 beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, at 560,422 to 561,850 15336 mRNA_6969: 15337 - K17261 CAP1_2, SRV2 adenylyl cyclase-associated protein, at 561,995 to 564,059 15337 mRNA_6970: 15338 - Hypothetical Protein, at 566,123 to 566,867 15338 mRNA_6971: 15339 - K03671 trxA thioredoxin 1, at 567,242 to 568,041 15339
Group Condition mRNA_6967 mRNA_6968 mRNA_6969 mRNA_6970 mRNA_6971
15335 15336 15337 15338 15339
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 -0.2 N.D. -0.3 -0.2
growth on oleic acid YNB Glucose -0.5 -0.2 N.D. -0.1 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.5 N.D. +0.6 -0.9
growth on oleic acid YNB Oleic Acid -0.1 -0.1 N.D. -0.1 -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.4 N.D. +0.1 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.4 N.D. +0.5 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 +0.1 N.D. -0.5 +0.6
auxotrophy YNB Glucose 75mM Methionine -0.6 -0.1 N.D. +0.7 -0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.1 N.D. -0.3 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -0.3 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Glucose -0.3 -0.1 N.D. +0.1 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.5 +0.1 N.D. +0.1 +0.2
auxotrophy Dropout Complete -0.1 -0.5 N.D. +0.5 +0.0
auxotrophy YNB Glucose -0.1 -0.2 N.D. +0.3 +0.0
growth on ypd YPD -0.5 +0.7 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.3 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 -0.3 N.D. +0.0 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.7 -0.1 N.D. -0.2 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.5 -0.2 N.D. +0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.3 N.D. +0.7 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.7 +0.1 N.D. +0.1 +0.6
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