Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15351 and 15352 are separated by 226 nucleotides15352 and 15353 are separated by 199 nucleotides15353 and 15354 are separated by 139 nucleotides15354 and 15355 are separated by 564 nucleotides mRNA_6983: 15351 - K02145 ATPeV1A, ATP6A V-type H+-transporting ATPase subunit A, at 596,836 to 599,468 15351 mRNA_6984: 15352 - HMMPfam-Fungal specific transcription factor domain-PF04082, at 599,695 to 602,665 15352 mRNA_6985: 15353 - BLAST chitin deacetylase [Moniliophthora roreri MCA 2997], at 602,865 to 604,394 15353 mRNA_6986: 15354 - K12872 RBM22, SLT11 pre-mRNA-splicing factor RBM22/SLT11, at 604,534 to 605,830 15354 mRNA_6987: 15355 - BLAST plasma membrane protein [Pseudozyma hubeiensis SY62], at 606,395 to 609,554 15355
Group Condition mRNA_6983 mRNA_6984 mRNA_6985 mRNA_6986 mRNA_6987
15351 15352 15353 15354 15355
auxotrophy YNB Glucose 75mM Methionine N.D. -0.9 +0.1 N.D. -0.4
growth on oleic acid YNB Glucose N.D. -0.8 -0.1 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.9 +0.2 N.D. +0.0
auxotrophy Dropout Complete N.D. -0.5 +0.1 N.D. -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 -0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. -0.7 +0.2 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.8 -0.2 N.D. +0.5
growth on oleic acid YNB Oleic Acid N.D. -0.4 +0.1 N.D. -0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.5 -0.1 N.D. +0.2
growth on ricinoleic acid YNB Glucose N.D. -0.6 +0.2 N.D. +0.1
growth on ypd YPD N.D. -0.1 -0.0 N.D. -0.1
auxotrophy YNB Glucose N.D. -0.6 +0.5 N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.0 N.D. +0.0
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.1 -0.0 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 +0.1 N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 -0.1 N.D. +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.0 -0.0 N.D. +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 -0.2 N.D. +0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.6 +0.1 N.D. -0.0
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