Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15358 and 15359 are separated by 643 nucleotides15359 and 15360 are separated by 352 nucleotides15360 and 15361 are separated by 80 nucleotides15361 and 15362 are separated by 126 nucleotides mRNA_6990: 15358 - K09001 anmK anhydro-N-acetylmuramic acid kinase, at 615,563 to 617,347 15358 mRNA_6991: 15359 - KOG0254 Predicted transporter (major facilitator superfamily), at 617,991 to 620,077 15359 mRNA_6992: 15360 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 620,430 to 622,573 15360 mRNA_6993: 15361 - HMMPfam-Protein of unknown function (DUF455)-PF04305,ProSiteProfiles-Rieske [2Fe-2S] iron-sulfur domain profile.-PS51296,SUPERFAMILY--SSF47240,SUPERFAMILY--SSF50022, at 622,654 to 624,401 15361 mRNA_6994: 15362 - K10134 EI24 etoposide-induced 2.4 mRNA, at 624,528 to 626,391 15362
Group Condition mRNA_6990 mRNA_6991 mRNA_6992 mRNA_6993 mRNA_6994
15358 15359 15360 15361 15362
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.6 -0.1 +0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.1 +0.1 -0.1 -0.6
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.3 -0.2 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 -0.0 -0.2 -0.3 +0.6
growth on oleic acid YNB Oleic Acid +0.2 -0.3 -0.1 -0.5 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.2 +0.1 -0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 +0.1 +0.0 -0.5
growth on oleic acid YNB Glucose -0.2 +0.1 -0.3 -0.1 +0.2
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.6 -0.3 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.2 +0.4 +0.0 -0.2 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 +0.0 -0.5 +0.1
growth on ypd YPD +0.1 +0.0 +0.2 -0.3 -0.1
ypd buoyancy 40 hrs Median Buoyancy -0.3 +0.1 +0.2 -0.1 +0.0
auxotrophy Dropout Complete +0.7 -0.0 +0.1 -0.8 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 +0.1 -0.4 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.4 +0.1 -0.8 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.8 -0.1 -0.3 -0.6 +0.4
growth on ricinoleic acid YNB Glucose +0.2 +0.3 +0.0 -0.2 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.8 +0.1 -0.2 -0.5 +0.6
auxotrophy YNB Glucose 75mM Arginine +0.5 +0.6 +0.4 -0.6 +0.2
auxotrophy YNB Glucose +0.6 +0.7 +0.2 -0.5 +0.3
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