Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15359 and 15360 are separated by 352 nucleotides15360 and 15361 are separated by 80 nucleotides15361 and 15362 are separated by 126 nucleotides15362 and 15363 are separated by 230 nucleotides mRNA_6991: 15359 - KOG0254 Predicted transporter (major facilitator superfamily), at 617,991 to 620,077 15359 mRNA_6992: 15360 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 620,430 to 622,573 15360 mRNA_6993: 15361 - HMMPfam-Protein of unknown function (DUF455)-PF04305,ProSiteProfiles-Rieske [2Fe-2S] iron-sulfur domain profile.-PS51296,SUPERFAMILY--SSF47240,SUPERFAMILY--SSF50022, at 622,654 to 624,401 15361 mRNA_6994: 15362 - K10134 EI24 etoposide-induced 2.4 mRNA, at 624,528 to 626,391 15362 mRNA_6995: 15363 - K15323 TSEN34 tRNA-splicing endonuclease subunit Sen34, at 626,622 to 627,882 15363
Group Condition mRNA_6991 mRNA_6992 mRNA_6993 mRNA_6994 mRNA_6995
15359 15360 15361 15362 15363
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -0.1 +0.2 -0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.3 -0.1 -0.5 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.2 -0.3 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.6 -0.3 +0.2 +0.1 N.D.
auxotrophy Dropout Complete -0.0 +0.1 -0.8 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.3 -0.6 +0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 +0.1 -0.1 -0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.0 -0.5 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.1 +0.0 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.1 -0.8 +0.0 N.D.
growth on ypd YPD +0.0 +0.2 -0.3 -0.1 N.D.
growth on oleic acid YNB Glucose +0.1 -0.3 -0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.1 -0.4 +0.0 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 +0.1 -0.2 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.2 -0.3 +0.6 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 -0.2 -0.5 +0.6 N.D.
growth on ricinoleic acid YNB Glucose +0.3 +0.0 -0.2 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 -0.1 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.6 +0.4 -0.6 +0.2 N.D.
auxotrophy YNB Glucose +0.7 +0.2 -0.5 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.0 -0.2 +0.6 N.D.
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