Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15360 and 15361 are separated by 80 nucleotides15361 and 15362 are separated by 126 nucleotides15362 and 15363 are separated by 230 nucleotides15363 and 15364 are separated by 126 nucleotides mRNA_6992: 15360 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 620,430 to 622,573 15360 mRNA_6993: 15361 - HMMPfam-Protein of unknown function (DUF455)-PF04305,ProSiteProfiles-Rieske [2Fe-2S] iron-sulfur domain profile.-PS51296,SUPERFAMILY--SSF47240,SUPERFAMILY--SSF50022, at 622,654 to 624,401 15361 mRNA_6994: 15362 - K10134 EI24 etoposide-induced 2.4 mRNA, at 624,528 to 626,391 15362 mRNA_6995: 15363 - K15323 TSEN34 tRNA-splicing endonuclease subunit Sen34, at 626,622 to 627,882 15363 mRNA_6996: 15364 - Hypothetical Protein, at 628,009 to 630,189 15364
Group Condition mRNA_6992 mRNA_6993 mRNA_6994 mRNA_6995 mRNA_6996
15360 15361 15362 15363 15364
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 -0.6 N.D. -0.3
auxotrophy Dropout Complete +0.1 -0.8 +0.1 N.D. +0.0
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.8 +0.0 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.6 +0.4 N.D. -0.0
growth on oleic acid YNB Oleic Acid -0.1 -0.5 +0.1 N.D. +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.0 -0.5 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 +0.1 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.2 -0.1 N.D. -0.1
growth on oleic acid YNB Glucose -0.3 -0.1 +0.2 N.D. -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.4 +0.0 N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.5 +0.1 N.D. +0.2
growth on ypd YPD +0.2 -0.3 -0.1 N.D. +0.1
growth on ricinoleic acid YNB Glucose +0.0 -0.2 +0.0 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.2 -0.3 N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 -0.1 +0.0 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.4 -0.6 +0.2 N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.5 +0.6 N.D. +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 +0.6 N.D. +0.2
auxotrophy YNB Glucose +0.2 -0.5 +0.3 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.2 +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.2 +0.6 N.D. +0.0
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