Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15362 and 15363 are separated by 230 nucleotides15363 and 15364 are separated by 126 nucleotides15364 and 15365 are separated by 64 nucleotides15365 and 15366 are separated by 238 nucleotides mRNA_6994: 15362 - K10134 EI24 etoposide-induced 2.4 mRNA, at 624,528 to 626,391 15362 mRNA_6995: 15363 - K15323 TSEN34 tRNA-splicing endonuclease subunit Sen34, at 626,622 to 627,882 15363 mRNA_6996: 15364 - Hypothetical Protein, at 628,009 to 630,189 15364 mRNA_6997: 15365 - K17053 ABP140 tRNAThr (cytosine32-N3)-methyltransferase, at 630,254 to 632,002 15365 mRNA_6998: 15366 - K00288 MTHFD methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase, at 632,241 to 635,924 15366
Group Condition mRNA_6994 mRNA_6995 mRNA_6996 mRNA_6997 mRNA_6998
15362 15363 15364 15365 15366
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 N.D. -0.3 -0.1 -0.4
growth on ricinoleic acid YNB Glucose +0.0 N.D. +0.1 +0.2 -1.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.6 N.D. +0.0 -1.0 -0.7
growth on oleic acid YNB Glucose +0.2 N.D. -0.0 +0.2 -1.4
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.2 +0.3 -1.4
auxotrophy YNB Glucose +0.3 N.D. +0.1 +0.1 -1.3
auxotrophy YNB Glucose 75mM Arginine +0.2 N.D. +0.2 +0.1 -1.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. +0.2 +0.3 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 N.D. +0.1 -0.2 -0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. -0.0 +0.5 -1.3
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. +0.2 +0.0 -0.7
ypd buoyancy 40 hrs High Buoyancy -0.5 N.D. -0.0 +0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. +0.2 +0.3 -0.8
auxotrophy Dropout Complete +0.1 N.D. +0.0 +0.3 -0.6
growth on ypd YPD -0.1 N.D. +0.1 +0.1 -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.1 +0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.1 +0.2 -0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. +0.1 -0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. +0.4 +0.2 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. +0.2 -0.2 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 N.D. +0.2 -0.0 -0.3
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