Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15363 and 15364 are separated by 126 nucleotides15364 and 15365 are separated by 64 nucleotides15365 and 15366 are separated by 238 nucleotides15366 and 15367 are separated by 339 nucleotides mRNA_6995: 15363 - K15323 TSEN34 tRNA-splicing endonuclease subunit Sen34, at 626,622 to 627,882 15363 mRNA_6996: 15364 - Hypothetical Protein, at 628,009 to 630,189 15364 mRNA_6997: 15365 - K17053 ABP140 tRNAThr (cytosine32-N3)-methyltransferase, at 630,254 to 632,002 15365 mRNA_6998: 15366 - K00288 MTHFD methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase, at 632,241 to 635,924 15366 mRNA_6999: 15367 - HMMPfam-CUE domain-PF02845,ProSiteProfiles-CUE domain profile.-PS51140,SMART-Domain that may be involved in binding ubiquitin-conjugating enzymes (UBCs)-SM00546,SUPERFAMILY--SSF46934, at 636,264 to 637,761 15367
Group Condition mRNA_6995 mRNA_6996 mRNA_6997 mRNA_6998 mRNA_6999
15363 15364 15365 15366 15367
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 -1.0 -0.7 +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 -0.1 -0.4 -0.6
growth on oleic acid YNB Glucose N.D. -0.0 +0.2 -1.4 +0.0
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.2 -1.3 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 +0.5 -1.3 +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 +0.3 -1.4 +0.4
auxotrophy YNB Glucose N.D. +0.1 +0.1 -1.3 +0.7
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.0 -0.7 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.2 -0.3 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.2 +0.1 -1.1 +0.5
growth on oleic acid YNB Oleic Acid N.D. +0.1 -0.1 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 -0.2 -0.1 +0.0
growth on ypd YPD N.D. +0.1 +0.1 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.0 -0.3 +0.1
auxotrophy Dropout Complete N.D. +0.0 +0.3 -0.6 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.1 +0.0 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.2 -0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 +0.1 +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.4 +0.2 -0.5 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 +0.3 -0.8 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 +0.3 -0.6 +0.6
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