Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15364 and 15365 are separated by 64 nucleotides15365 and 15366 are separated by 238 nucleotides15366 and 15367 are separated by 339 nucleotides15367 and 15368 are separated by 604 nucleotides mRNA_6996: 15364 - Hypothetical Protein, at 628,009 to 630,189 15364 mRNA_6997: 15365 - K17053 ABP140 tRNAThr (cytosine32-N3)-methyltransferase, at 630,254 to 632,002 15365 mRNA_6998: 15366 - K00288 MTHFD methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase, at 632,241 to 635,924 15366 mRNA_6999: 15367 - HMMPfam-CUE domain-PF02845,ProSiteProfiles-CUE domain profile.-PS51140,SMART-Domain that may be involved in binding ubiquitin-conjugating enzymes (UBCs)-SM00546,SUPERFAMILY--SSF46934, at 636,264 to 637,761 15367 mRNA_7000: 15368 - K06653 PHO81 CDK inhibitor PHO81, at 638,366 to 642,076 15368
Group Condition mRNA_6996 mRNA_6997 mRNA_6998 mRNA_6999 mRNA_7000
15364 15365 15366 15367 15368
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -0.1 -0.4 -0.6 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -1.0 -0.7 +0.2 -0.2
growth on oleic acid YNB Glucose -0.0 +0.2 -1.4 +0.0 +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 +0.0 -0.1 -0.6
growth on ricinoleic acid YNB Glucose +0.1 +0.2 -1.3 +0.4 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 +0.5 -1.3 +0.2 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.2 -0.3 +0.1 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 -0.1 +0.1 -0.2
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.3 -1.4 +0.4 +0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.0 -0.7 +0.1 +0.4
growth on ypd YPD +0.1 +0.1 -0.1 -0.1 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 -1.1 +0.5 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 +0.3 -0.8 +0.5 +0.1
auxotrophy YNB Glucose +0.1 +0.1 -1.3 +0.7 +0.8
growth on oleic acid YNB Oleic Acid +0.1 -0.1 -0.1 -0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.4 +0.2 -0.5 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.2 -0.1 +0.0 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.0 -0.3 +0.1 +0.5
ypd buoyancy 40 hrs High Buoyancy -0.0 +0.1 +0.1 -0.1 +0.4
auxotrophy Dropout Complete +0.0 +0.3 -0.6 +0.3 +0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.3 -0.6 +0.6 +0.6
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