Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15381 and 15382 are separated by 2103 nucleotides15382 and 15383 are separated by 1470 nucleotides15383 and 15384 are separated by 153 nucleotides15384 and 15385 are separated by 113 nucleotides mRNA_7013: 15381 - K10755 RFC2_4 replication factor C subunit 2/4, at 672,641 to 674,296 15381 mRNA_7014: 15382 - KOG1721 FOG Zn-finger, at 676,400 to 676,740 15382 mRNA_7015: 15383 - KOG2913 Predicted membrane protein, at 678,211 to 680,397 15383 mRNA_7016: 15384 - K01142 E3.1.11.2, xthA exodeoxyribonuclease III, at 680,551 to 683,526 15384 mRNA_7017: 15385 - K03574 mutT, NUDT15, MTH2 8-oxo-dGTP diphosphatase, at 683,640 to 684,233 15385
Group Condition mRNA_7013 mRNA_7014 mRNA_7015 mRNA_7016 mRNA_7017
15381 15382 15383 15384 15385
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.4 -0.2 -1.6
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.1 +0.4 -2.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.3 -0.3 -1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.5 -0.2 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 -0.2 -0.5
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.0 +0.1 -0.6
growth on ypd YPD N.D. N.D. +0.3 +0.2 -0.9
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 +0.2 -0.5
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.3 +0.1 -0.6
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.2 +0.1 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.1 +0.1 +0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.2 +0.1 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.6 -0.0 -0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.4 +0.1 -0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.3 +0.1 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.3 -0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 +0.2 +0.1
auxotrophy Dropout Complete N.D. N.D. +0.3 +0.1 +0.5
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.4 +0.1 +0.5
growth on oleic acid YNB Glucose N.D. N.D. +0.3 +0.1 +0.6
auxotrophy YNB Glucose N.D. N.D. +0.2 +0.3 +0.8
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