Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15382 and 15383 are separated by 1470 nucleotides15383 and 15384 are separated by 153 nucleotides15384 and 15385 are separated by 113 nucleotides15385 and 15386 are separated by 583 nucleotides mRNA_7014: 15382 - KOG1721 FOG Zn-finger, at 676,400 to 676,740 15382 mRNA_7015: 15383 - KOG2913 Predicted membrane protein, at 678,211 to 680,397 15383 mRNA_7016: 15384 - K01142 E3.1.11.2, xthA exodeoxyribonuclease III, at 680,551 to 683,526 15384 mRNA_7017: 15385 - K03574 mutT, NUDT15, MTH2 8-oxo-dGTP diphosphatase, at 683,640 to 684,233 15385 mRNA_7018: 15386 - Hypothetical Protein, at 684,817 to 685,871 15386
Group Condition mRNA_7014 mRNA_7015 mRNA_7016 mRNA_7017 mRNA_7018
15382 15383 15384 15385 15386
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.1 +0.4 -2.4 -0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.4 -0.2 -1.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.5 -0.2 -0.5 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.3 -0.3 -1.0 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.2 -0.5 -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.1 -0.6 -0.0
growth on ypd YPD N.D. +0.3 +0.2 -0.9 +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.1 -0.6 +0.2
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 +0.2 -0.5 +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.1 +0.0 -0.0
growth on oleic acid YNB Oleic Acid N.D. +0.2 +0.1 -0.1 +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.2 +0.1 -0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.6 -0.0 -0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.4 +0.1 -0.1 +0.2
auxotrophy Dropout Complete N.D. +0.3 +0.1 +0.5 -0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 -0.0 +0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.3 +0.1 +0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 +0.2 +0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 +0.1 +0.5 +0.2
growth on oleic acid YNB Glucose N.D. +0.3 +0.1 +0.6 +0.3
auxotrophy YNB Glucose N.D. +0.2 +0.3 +0.8 +0.2
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