Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15393 and 15394 are separated by 182 nucleotides15394 and 15395 are separated by 263 nucleotides15395 and 15396 are separated by 310 nucleotides15396 and 15397 are separated by 256 nucleotides mRNA_7025: 15393 - K13122 FRG1 protein FRG1, at 701,812 to 702,904 15393 mRNA_7026: 15394 - K12827 SF3A3, SAP61, PRP9 splicing factor 3A subunit 3, at 703,087 to 704,939 15394 mRNA_7027: 15395 - K13025 EIF4A3, FAL1 ATP-dependent RNA helicase, at 705,203 to 707,269 15395 mRNA_7028: 15396 - SUPERFAMILY--SSF81383, at 707,580 to 708,539 15396 mRNA_7029: 15397 - Hypothetical Protein, at 708,796 to 709,745 15397
Group Condition mRNA_7025 mRNA_7026 mRNA_7027 mRNA_7028 mRNA_7029
15393 15394 15395 15396 15397
auxotrophy YNB Glucose 75mM Methionine -0.8 N.D. N.D. N.D. -1.0
growth on ricinoleic acid YNB Ricinoleic Acid -1.7 N.D. N.D. N.D. +0.3
auxotrophy YNB Glucose -0.6 N.D. N.D. N.D. -0.6
growth on ypd YPD -1.0 N.D. N.D. N.D. -0.2
auxotrophy YNB Glucose 75mM Arginine -0.8 N.D. N.D. N.D. -0.2
growth on ricinoleic acid YNB Glucose -0.9 N.D. N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -1.2 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 N.D. N.D. N.D. -0.6
auxotrophy Dropout Complete -0.5 N.D. N.D. N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. N.D. N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.7 N.D. N.D. N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.7 N.D. N.D. N.D. +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.7 N.D. N.D. N.D. +0.3
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.2 N.D. N.D. N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. N.D. N.D. -0.0
growth on oleic acid YNB Glucose -0.4 N.D. N.D. N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 N.D. N.D. N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. N.D. N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 N.D. N.D. N.D. +0.1
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