Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15403 and 15404 are separated by 874 nucleotides15404 and 15405 are separated by 120 nucleotides15405 and 15406 are separated by 548 nucleotides15406 and 15407 are separated by 350 nucleotides mRNA_7035: 15403 - K11826 AP2M1 AP-2 complex subunit mu-1, at 718,322 to 720,163 15403 mRNA_7036: 15404 - K00831 serC, PSAT1 phosphoserine aminotransferase, at 721,038 to 722,425 15404 mRNA_7037: 15405 - K08254 E3.2.1.59 glucan endo-1,3-alpha-glucosidase, at 722,546 to 724,803 15405 mRNA_7038: 15406 - K15458 SAP185_190 SIT4-associating protein SAP185/190, at 725,352 to 729,605 15406 mRNA_7039: 15407 - Hypothetical Protein, at 729,956 to 731,972 15407
Group Condition mRNA_7035 mRNA_7036 mRNA_7037 mRNA_7038 mRNA_7039
15403 15404 15405 15406 15407
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 -1.0 -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 -0.5 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.6 -0.3 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.5 -0.4 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.3 -0.5 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.3 -0.4 +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.2 -0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.5 -0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.0 -0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.0 -0.1
growth on ypd YPD N.D. N.D. +0.5 -0.4 -0.3
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 -0.2 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.2 -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. -0.2 +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.3 +0.3 -0.0
growth on oleic acid YNB Glucose N.D. N.D. +0.0 -0.4 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 +0.3 -0.3
auxotrophy Dropout Complete N.D. N.D. -0.0 -0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.2 -0.1 -0.1
auxotrophy YNB Glucose N.D. N.D. -0.1 -0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.0 -0.0 +0.4
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