Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15411 and 15412 are separated by 194 nucleotides15412 and 15413 are separated by 1366 nucleotides15413 and 15414 are separated by 151 nucleotides15414 and 15415 are separated by 388 nucleotides mRNA_7043: 15411 - Hypothetical Protein, at 736,926 to 740,766 15411 mRNA_7044: 15412 - KOG4299 PHD Zn-finger protein, at 740,961 to 743,698 15412 mRNA_7045: 15413 - K20288 COG1 conserved oligomeric Golgi complex subunit 1, at 745,065 to 748,525 15413 mRNA_7046: 15414 - K01341 KEX2 kexin, at 748,677 to 751,864 15414 mRNA_7047: 15415 - K07466 RFA1, RPA1, rpa replication factor A1, at 752,253 to 754,600 15415
Group Condition mRNA_7043 mRNA_7044 mRNA_7045 mRNA_7046 mRNA_7047
15411 15412 15413 15414 15415
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -2.1 N.D. +0.0 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 -1.1 N.D. -0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.9 N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.6 +0.1 N.D. -0.4 N.D.
growth on ypd YPD -0.1 +0.1 N.D. -0.8 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.4 N.D. -1.0 N.D.
growth on oleic acid YNB Oleic Acid +0.1 +0.2 N.D. -0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.0 -0.8 N.D. +0.8 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.0 +0.1 N.D. -0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 +0.5 N.D. +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.0 N.D. +0.8 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.2 N.D. +0.9 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.2 N.D. +0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +1.1 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose -0.1 +0.9 N.D. +0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +1.4 N.D. -0.2 N.D.
auxotrophy Dropout Complete -0.1 +1.0 N.D. +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 +0.9 N.D. +0.3 N.D.
growth on ricinoleic acid YNB Glucose +0.1 +1.0 N.D. +0.5 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.3 +0.9 N.D. +0.3 N.D.
auxotrophy YNB Glucose +0.3 +1.2 N.D. +0.7 N.D.
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