Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15413 and 15414 are separated by 151 nucleotides15414 and 15415 are separated by 388 nucleotides15415 and 15416 are separated by 378 nucleotides15416 and 15417 are separated by 1118 nucleotides mRNA_7045: 15413 - K20288 COG1 conserved oligomeric Golgi complex subunit 1, at 745,065 to 748,525 15413 mRNA_7046: 15414 - K01341 KEX2 kexin, at 748,677 to 751,864 15414 mRNA_7047: 15415 - K07466 RFA1, RPA1, rpa replication factor A1, at 752,253 to 754,600 15415 mRNA_7048: 15416 - KOG2476 Uncharacterized conserved protein, at 754,979 to 757,509 15416 mRNA_7049: 15417 - KOG0565 Inositol polyphosphate 5-phosphatase and related proteins, at 758,628 to 760,981 15417
Group Condition mRNA_7045 mRNA_7046 mRNA_7047 mRNA_7048 mRNA_7049
15413 15414 15415 15416 15417
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 N.D. +0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 N.D. -0.1 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 N.D. -0.2 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.5 N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 N.D. +0.1 -0.2
auxotrophy Dropout Complete N.D. +0.3 N.D. +0.2 -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. +0.3 N.D. +0.3 -0.7
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 N.D. +0.3 -0.3
growth on ypd YPD N.D. -0.8 N.D. +0.3 +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. +0.3 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.0 N.D. +0.5 -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 N.D. +0.3 -0.4
growth on ricinoleic acid YNB Glucose N.D. +0.5 N.D. +0.4 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.4 -0.0
growth on oleic acid YNB Glucose N.D. +0.3 N.D. +0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.8 N.D. +0.1 -0.5
auxotrophy YNB Glucose N.D. +0.7 N.D. +0.5 -0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 N.D. +0.3 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.6 N.D. +0.3 +0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.8 N.D. +0.3 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.9 N.D. +0.1 +0.4
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