Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15420 and 15421 are separated by 286 nucleotides15421 and 15422 are separated by 233 nucleotides15422 and 15423 are separated by 278 nucleotides15423 and 15424 are separated by 521 nucleotides mRNA_7052: 15420 - K01624 FBA, fbaA fructose-bisphosphate aldolase, class II, at 764,515 to 766,298 15420 mRNA_7053: 15421 - K01192 E3.2.1.25, MANBA, manB beta-mannosidase, at 766,585 to 770,373 15421 mRNA_7054: 15422 - K14018 PLAA, DOA1, UFD3 phospholipase A-2-activating protein, at 770,607 to 773,938 15422 mRNA_7055: 15423 - K03841 FBP, fbp fructose-1,6-bisphosphatase I, at 774,217 to 775,850 15423 mRNA_7056: 15424 - K01557 FAHD1 acylpyruvate hydrolase, at 776,372 to 777,538 15424
Group Condition mRNA_7052 mRNA_7053 mRNA_7054 mRNA_7055 mRNA_7056
15420 15421 15422 15423 15424
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.3 N.D. -0.7 -1.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.0 N.D. -1.9 +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. -1.0 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 N.D. -1.9 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. -0.3 -0.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 N.D. -0.0 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 N.D. -0.0 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 N.D. -0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.0 -0.4
growth on oleic acid YNB Glucose N.D. -0.1 N.D. +0.1 -0.1
auxotrophy Dropout Complete N.D. +0.0 N.D. -0.0 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 N.D. -0.0 -0.1
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 N.D. +0.2 +0.1
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. +0.1 +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 N.D. +0.4 +0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.3 N.D. +0.5 +0.2
growth on ricinoleic acid YNB Glucose N.D. -0.0 N.D. +0.1 +0.4
auxotrophy YNB Glucose N.D. +0.4 N.D. +0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.4 N.D. +0.5 +0.7
growth on ypd YPD N.D. +0.5 N.D. +0.2 +0.3
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