Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15430 and 15431 are separated by 11 nucleotides15431 and 15432 are separated by 1722 nucleotides15432 and 15433 are separated by 158 nucleotides15433 and 15434 are separated by 277 nucleotides mRNA_7062: 15430 - HMMPfam-N-acetyltransferase-PF00797,SUPERFAMILY--SSF54001, at 792,528 to 793,710 15430 mRNA_7063: 15431 - Hypothetical Protein, at 793,722 to 794,198 15431 mRNA_7064: 15432 - K12778 HOP1 meiosis-specific protein HOP1, at 795,921 to 797,535 15432 mRNA_7065: 15433 - K03469 rnhA, RNASEH1 ribonuclease HI, at 797,694 to 798,933 15433 mRNA_7066: 15434 - K00799 GST, gst glutathione S-transferase, at 799,211 to 800,128 15434
Group Condition mRNA_7062 mRNA_7063 mRNA_7064 mRNA_7065 mRNA_7066
15430 15431 15432 15433 15434
growth on ypd YPD -0.4 -0.5 -0.1 -0.0 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.5 +0.3 +0.2 -0.6
growth on oleic acid YNB Oleic Acid -1.0 -0.4 +0.0 +0.4 +0.7
ypd buoyancy 40 hrs Low Buoyancy -0.6 +1.0 -0.2 -0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.3 +0.3 -0.4 +0.4
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.3 -0.3 -0.1 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.1 -0.2 +0.2 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.8 +0.4 +0.4 -0.6 -0.8
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.4 +0.4 +0.3 +0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 +0.5 -0.0 -0.5 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 +1.2 +0.4 -0.6 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.2 +1.3 -0.1 +0.0 +0.4
growth on oleic acid YNB Glucose +0.0 +0.1 -0.4 +0.3 +0.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.8 +0.3 -0.5 +0.5
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.4 -0.0 +0.1 +0.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.5 +0.7 -0.0 -0.2 +0.8
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.3 +0.6 +0.2 -0.1
auxotrophy Dropout Complete +0.3 +0.2 +0.1 +0.2 +0.5
auxotrophy YNB Glucose +0.0 +0.1 +0.4 +0.5 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.7 +1.2 +0.2 +0.2 -0.6
growth on ricinoleic acid YNB Glucose +0.9 +0.4 +0.3 -0.2 +0.3
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