Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15432 and 15433 are separated by 158 nucleotides15433 and 15434 are separated by 277 nucleotides15434 and 15435 are separated by 404 nucleotides15435 and 15436 are separated by 477 nucleotides mRNA_7064: 15432 - K12778 HOP1 meiosis-specific protein HOP1, at 795,921 to 797,535 15432 mRNA_7065: 15433 - K03469 rnhA, RNASEH1 ribonuclease HI, at 797,694 to 798,933 15433 mRNA_7066: 15434 - K00799 GST, gst glutathione S-transferase, at 799,211 to 800,128 15434 mRNA_7067: 15435 - K13507 GAT glycerol-3-phosphate O-acyltransferase / dihydroxyacetone phosphate acyltransferase, at 800,533 to 803,766 15435 mRNA_7068: 15436 - K00167 BCKDHB, bkdA2 2-oxoisovalerate dehydrogenase E1 component beta subunit, at 804,244 to 806,337 15436
Group Condition mRNA_7064 mRNA_7065 mRNA_7066 mRNA_7067 mRNA_7068
15432 15433 15434 15435 15436
facs on lipid accumulation Sorted High BODIPY Gate +0.4 -0.6 -0.8 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 -0.5 N.D. +0.3
growth on ypd YPD -0.1 -0.0 +0.1 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 -0.6 -0.1 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.5 +0.5 N.D. -0.5
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 -0.5 -0.3 N.D. +0.7
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.2 -0.6 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 +0.1 N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 +0.4 N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.4 +0.4 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.2 +0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.2 -0.6 N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.2 +0.8 N.D. -0.0
growth on oleic acid YNB Glucose -0.4 +0.3 +0.7 N.D. +0.0
auxotrophy Dropout Complete +0.1 +0.2 +0.5 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.3 -0.2 +0.3 N.D. +0.3
growth on oleic acid YNB Oleic Acid +0.0 +0.4 +0.7 N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 +0.6 N.D. +0.3
auxotrophy YNB Glucose 75mM Methionine +0.4 +0.3 +0.5 N.D. -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.6 +0.2 -0.1 N.D. +0.4
auxotrophy YNB Glucose +0.4 +0.5 +0.3 N.D. +0.3
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