Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15433 and 15434 are separated by 277 nucleotides15434 and 15435 are separated by 404 nucleotides15435 and 15436 are separated by 477 nucleotides15436 and 15437 are separated by 159 nucleotides mRNA_7065: 15433 - K03469 rnhA, RNASEH1 ribonuclease HI, at 797,694 to 798,933 15433 mRNA_7066: 15434 - K00799 GST, gst glutathione S-transferase, at 799,211 to 800,128 15434 mRNA_7067: 15435 - K13507 GAT glycerol-3-phosphate O-acyltransferase / dihydroxyacetone phosphate acyltransferase, at 800,533 to 803,766 15435 mRNA_7068: 15436 - K00167 BCKDHB, bkdA2 2-oxoisovalerate dehydrogenase E1 component beta subunit, at 804,244 to 806,337 15436 mRNA_7069: 15437 - HMMPfam-DNA-directed RNA polymerase III subunit Rpc31-PF11705, at 806,497 to 812,015 15437
Group Condition mRNA_7065 mRNA_7066 mRNA_7067 mRNA_7068 mRNA_7069
15433 15434 15435 15436 15437
facs on lipid accumulation Sorted High BODIPY Gate -0.6 -0.8 N.D. +0.0 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 -0.1 N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 +0.5 N.D. -0.5 +0.1
growth on ypd YPD -0.0 +0.1 N.D. -0.4 -0.1
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.3 N.D. +0.7 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.5 N.D. +0.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.4 +0.4 N.D. +0.0 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.6 N.D. +0.5 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.6 N.D. +0.2 +0.3
growth on ricinoleic acid YNB Glucose -0.2 +0.3 N.D. +0.3 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.4 N.D. -0.1 +0.0
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.1 N.D. +0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.1 N.D. +0.4 +0.1
auxotrophy Dropout Complete +0.2 +0.5 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 N.D. +0.4 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.3 +0.5 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 +0.8 N.D. -0.0 +0.2
growth on oleic acid YNB Oleic Acid +0.4 +0.7 N.D. -0.1 -0.1
growth on oleic acid YNB Glucose +0.3 +0.7 N.D. +0.0 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.6 N.D. +0.3 +0.1
auxotrophy YNB Glucose +0.5 +0.3 N.D. +0.3 +0.1
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