Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15435 and 15436 are separated by 477 nucleotides15436 and 15437 are separated by 159 nucleotides15437 and 15438 are separated by 657 nucleotides15438 and 15439 are separated by 370 nucleotides mRNA_7067: 15435 - K13507 GAT glycerol-3-phosphate O-acyltransferase / dihydroxyacetone phosphate acyltransferase, at 800,533 to 803,766 15435 mRNA_7068: 15436 - K00167 BCKDHB, bkdA2 2-oxoisovalerate dehydrogenase E1 component beta subunit, at 804,244 to 806,337 15436 mRNA_7069: 15437 - HMMPfam-DNA-directed RNA polymerase III subunit Rpc31-PF11705, at 806,497 to 812,015 15437 mRNA_7070: 15438 - K13953 adhP alcohol dehydrogenase, propanol-preferring, at 812,673 to 814,209 15438 mRNA_7071: 15439 - BLAST histone H3 h3.1 [Schizosaccharomyces japonicus yF..., at 814,580 to 815,327 15439
Group Condition mRNA_7067 mRNA_7068 mRNA_7069 mRNA_7070 mRNA_7071
15435 15436 15437 15438 15439
growth on ypd YPD N.D. -0.4 -0.1 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 -0.0 -0.2 +0.5
auxotrophy YNB Glucose 75mM Methionine N.D. -0.0 -0.1 +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.5 +0.1 -0.0 +0.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.0 -0.0 +0.1 +0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 +0.3 +0.4 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.0 +0.2 +0.4
auxotrophy Dropout Complete N.D. -0.1 -0.0 +0.4 +0.3
growth on oleic acid YNB Glucose N.D. +0.0 -0.0 -0.1 +0.6
auxotrophy YNB Glucose 75mM Arginine N.D. +0.3 +0.1 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 +0.2 +0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.3 -0.1 +0.3 +0.2
auxotrophy YNB Glucose N.D. +0.3 +0.1 +0.2 +0.3
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.1 -0.0 +1.5
ypd buoyancy 40 hrs High Buoyancy N.D. +0.4 +0.1 +0.2 +0.5
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 -0.0 +0.1 +1.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.0 +0.2 +0.8
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.1 +0.2 +1.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.7 -0.2 +0.6 +0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.5 -0.1 -0.0 +2.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 -0.1 +0.1 +2.2
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