Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15437 and 15438 are separated by 657 nucleotides15438 and 15439 are separated by 370 nucleotides15439 and 15440 are separated by 286 nucleotides15440 and 15441 are separated by 595 nucleotides mRNA_7069: 15437 - HMMPfam-DNA-directed RNA polymerase III subunit Rpc31-PF11705, at 806,497 to 812,015 15437 mRNA_7070: 15438 - K13953 adhP alcohol dehydrogenase, propanol-preferring, at 812,673 to 814,209 15438 mRNA_7071: 15439 - BLAST histone H3 h3.1 [Schizosaccharomyces japonicus yF..., at 814,580 to 815,327 15439 mRNA_7072: 15440 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 815,614 to 818,476 15440 mRNA_7073: 15441 - HMMPfam-Domain of unknown function DUF77-PF01910,SUPERFAMILY--SSF89957,TIGRFAM-TIGR00106 uncharacterized protein, MTH1187 family-TIGR00106, at 819,072 to 820,252 15441
Group Condition mRNA_7069 mRNA_7070 mRNA_7071 mRNA_7072 mRNA_7073
15437 15438 15439 15440 15441
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 +0.6 +0.9 -0.6 -2.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.4 -0.4 -0.5 -0.1
auxotrophy Dropout Complete -0.0 +0.4 +0.3 +0.1 -0.9
auxotrophy YNB Glucose +0.1 +0.2 +0.3 +0.0 -0.8
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.1 +0.4 -0.4 -0.1
growth on ypd YPD -0.1 -0.2 -0.0 +0.5 +0.0
growth on oleic acid YNB Glucose -0.0 -0.1 +0.6 +0.3 -0.5
facs on lipid accumulation Sorted No BODIPY Gate -0.0 +0.1 +0.3 -0.4 +0.3
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.2 +0.8 -0.0 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.0 +0.8 -0.2 -0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 -0.2 +0.5 +0.0 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.3 +0.2 +0.0 +0.3
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 +0.1 +0.2 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 +0.5 -0.2 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.0 +0.1 +1.1 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.2 +0.4 +0.1 +0.6
growth on oleic acid YNB Oleic Acid -0.1 -0.0 +1.5 +0.4 -0.5
growth on ricinoleic acid YNB Glucose -0.1 +0.2 +1.1 +0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.2 +0.2 +0.5 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.0 +2.3 +0.8 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 +2.2 +0.7 +0.9
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