Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15449 and 15450 are separated by 112 nucleotides15450 and 15451 are separated by 7 nucleotides15451 and 15452 are separated by 149 nucleotides15452 and 15453 are separated by 164 nucleotides mRNA_7081: 15449 - K00272 DDO D-aspartate oxidase, at 836,490 to 837,896 15449 mRNA_7082: 15450 - K20406 NPRL3 nitrogen permease regulator 3-like protein, at 838,009 to 840,569 15450 mRNA_7083: 15451 - K18803 HPM1 protein-histidine N-methyltransferase, at 840,577 to 841,991 15451 mRNA_7084: 15452 - KOG1575 Voltage-gated shaker-like K+ channel, subunit beta/KCNAB, at 842,141 to 843,806 15452 mRNA_7085: 15453 - HMMPfam-TRF2-interacting telomeric protein/Rap1 - C terminal domain-PF11626,SUPERFAMILY--SSF46689, at 843,971 to 847,409 15453
Group Condition mRNA_7081 mRNA_7082 mRNA_7083 mRNA_7084 mRNA_7085
15449 15450 15451 15452 15453
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.8 -0.6 -2.8 +0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.9 -0.2 -2.1 +0.1 -1.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 -0.4 +0.1 -0.4 -1.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 -0.4 -1.2 +0.3 -0.8
auxotrophy Dropout Complete -0.0 -0.6 -0.3 +0.3 -1.7
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -1.1 -1.5 +0.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -1.3 +0.2 +0.2 -0.9
growth on ricinoleic acid YNB Glucose -0.3 +0.3 -0.2 -0.2 -1.0
auxotrophy YNB Glucose 75mM Methionine +0.5 -1.1 -0.9 -0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.3 -0.4 -0.1 -0.3
auxotrophy YNB Glucose +0.3 -0.5 -0.1 +0.4 -0.7
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.0 -0.6 -0.2 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.7 -0.4 -0.3 -0.1 +1.1
growth on oleic acid YNB Glucose +0.2 +0.2 +0.4 -0.2 -0.6
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.2 +0.4 +0.0 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.4 +0.9 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.2 -0.6 +0.1 +0.9
growth on ypd YPD -0.2 -0.1 -1.8 +0.2 +2.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.0 +1.1 -0.2 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.4 +0.7 +0.4 +0.3
growth on oleic acid YNB Oleic Acid +0.4 -0.4 +1.3 -0.1 +0.8
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