Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15460 and 15461 are separated by 358 nucleotides15461 and 15462 are separated by 1789 nucleotides15462 and 15463 are separated by 26 nucleotides15463 and 15464 are separated by 287 nucleotides mRNA_7092: 15460 - KOG2545 Conserved membrane protein, at 863,308 to 866,540 15460 mRNA_7093: 15461 - K16073 ALR, MNR magnesium transporter, at 866,899 to 868,705 15461 mRNA_7094: 15462 - KOG1667 Zn2+-binding protein Melusin/RAR1, contains CHORD domain, at 870,495 to 871,178 15462 mRNA_7095: 15463 - Hypothetical Protein, at 871,205 to 873,737 15463 mRNA_7096: 15464 - K16729 CHORDC1, CHP1 cysteine and histidine-rich domain-containing protein 1, at 874,025 to 875,519 15464
Group Condition mRNA_7092 mRNA_7093 mRNA_7094 mRNA_7095 mRNA_7096
15460 15461 15462 15463 15464
auxotrophy Dropout Complete +0.2 N.D. N.D. -1.5 -0.7
auxotrophy YNB Glucose 75mM Arginine -0.1 N.D. N.D. -1.5 -0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.6 N.D. N.D. -1.1 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 N.D. N.D. -1.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 N.D. N.D. -1.0 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. N.D. -1.1 -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.0 N.D. N.D. -0.9 -0.5
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. N.D. -1.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. N.D. +0.4 -1.5
auxotrophy YNB Glucose +0.1 N.D. N.D. +0.3 -1.2
growth on ricinoleic acid YNB Glucose -0.1 N.D. N.D. +0.4 -0.6
growth on oleic acid YNB Glucose +0.2 N.D. N.D. -0.7 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. N.D. -0.5 +0.1
growth on oleic acid YNB Oleic Acid +0.2 N.D. N.D. -0.1 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 N.D. N.D. +0.3 -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 N.D. N.D. +1.4 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. N.D. +0.4 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. N.D. +1.0 -0.1
growth on ypd YPD -0.3 N.D. N.D. +0.4 +1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. +1.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 N.D. N.D. +1.7 -0.1
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