Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15465 and 15466 are separated by 225 nucleotides15466 and 15467 are separated by 382 nucleotides15467 and 15468 are separated by 375 nucleotides15468 and 15469 are separated by 97 nucleotides mRNA_7097: 15465 - K03064 PSMC6, RPT4 26S proteasome regulatory subunit T4, at 875,663 to 877,708 15465 mRNA_7098: 15466 - KOG2633 Hismacro and SEC14 domain-containing proteins, at 877,934 to 879,350 15466 mRNA_7099: 15467 - K12845 SNU13, NHP2L U4/U6 small nuclear ribonucleoprotein SNU13, at 879,733 to 880,505 15467 mRNA_7100: 15468 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 880,881 to 881,621 15468 mRNA_7101: 15469 - K08968 E1.8.4.14 L-methionine (R)-S-oxide reductase, at 881,719 to 882,426 15469
Group Condition mRNA_7097 mRNA_7098 mRNA_7099 mRNA_7100 mRNA_7101
15465 15466 15467 15468 15469
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 N.D. -0.4 -0.0
growth on oleic acid YNB Oleic Acid N.D. -0.1 N.D. -0.3 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. -0.0 N.D. -0.5 -0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.1 N.D. -0.5 -0.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.0 N.D. -0.3 -0.2
growth on ypd YPD N.D. +0.2 N.D. -0.4 -0.0
auxotrophy Dropout Complete N.D. +0.1 N.D. -0.3 -0.0
growth on oleic acid YNB Glucose N.D. -0.0 N.D. -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 N.D. +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.5 N.D. +0.2 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 N.D. -0.1 -0.0
growth on ricinoleic acid YNB Glucose N.D. -0.3 N.D. +0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.2 N.D. +0.1 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.2 N.D. -0.0 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 N.D. +0.2 -0.0
auxotrophy YNB Glucose N.D. -0.0 N.D. +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.3 N.D. +0.4 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.1 N.D. +0.4 -0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.2 N.D. +0.6 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.2 N.D. +0.9 -0.4
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