Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15467 and 15468 are separated by 375 nucleotides15468 and 15469 are separated by 97 nucleotides15469 and 15470 are separated by 454 nucleotides15470 and 15471 are separated by 1249 nucleotides mRNA_7099: 15467 - K12845 SNU13, NHP2L U4/U6 small nuclear ribonucleoprotein SNU13, at 879,733 to 880,505 15467 mRNA_7100: 15468 - BLAST putative protein [Melanopsichium pennsylvanicum 4], at 880,881 to 881,621 15468 mRNA_7101: 15469 - K08968 E1.8.4.14 L-methionine (R)-S-oxide reductase, at 881,719 to 882,426 15469 mRNA_7102: 15470 - K03441 GLP-F aquaglyceroporin related protein, other eukaryote, at 882,881 to 885,755 15470 mRNA_7103: 15471 - Hypothetical Protein, at 887,005 to 887,880 15471
Group Condition mRNA_7099 mRNA_7100 mRNA_7101 mRNA_7102 mRNA_7103
15467 15468 15469 15470 15471
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 +0.2 -0.4 -3.5
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.4 -0.0 +0.2 -2.7
growth on ypd YPD N.D. -0.4 -0.0 -0.1 -1.6
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.9 -0.4 -0.3 -1.8
facs on lipid accumulation Sorted No BODIPY Gate N.D. +0.4 -0.1 +0.1 -1.5
auxotrophy YNB Glucose 75mM Methionine N.D. -0.3 -0.2 +0.1 -0.4
auxotrophy Dropout Complete N.D. -0.3 -0.0 -0.1 +0.0
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.2 +0.2 -0.1 -0.8
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -0.3 -0.0 -0.1
growth on oleic acid YNB Glucose N.D. -0.0 -0.1 +0.3 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.0 -0.2 +0.4
ypd buoyancy 40 hrs High Buoyancy N.D. -0.5 -0.1 +0.3 +0.6
growth on ricinoleic acid YNB Glucose N.D. +0.2 +0.3 +0.1 -0.2
auxotrophy YNB Glucose N.D. +0.2 +0.1 +0.1 +0.0
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.3 +0.2 +1.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.0 +0.1 +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. -0.1 -0.0 +0.1 +0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 +0.0 -0.0 +0.8
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.5 -0.1 +0.4 +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.6 -0.1 -0.0 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 -0.0 +0.2 +1.3
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