Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15469 and 15470 are separated by 454 nucleotides15470 and 15471 are separated by 1249 nucleotides15471 and 15472 are separated by 552 nucleotides15472 and 15473 are separated by 422 nucleotides mRNA_7101: 15469 - K08968 E1.8.4.14 L-methionine (R)-S-oxide reductase, at 881,719 to 882,426 15469 mRNA_7102: 15470 - K03441 GLP-F aquaglyceroporin related protein, other eukaryote, at 882,881 to 885,755 15470 mRNA_7103: 15471 - Hypothetical Protein, at 887,005 to 887,880 15471 mRNA_7104: 15472 - K09448 TEAD transcriptional enhancer factor, at 888,433 to 891,786 15472 mRNA_7105: 15473 - KOG1890 Phosphoinositide phosphatase SAC1, at 892,209 to 895,612 15473
Group Condition mRNA_7101 mRNA_7102 mRNA_7103 mRNA_7104 mRNA_7105
15469 15470 15471 15472 15473
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 -3.5 +0.4 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.4 -0.3 -1.8 -0.3 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.0 +0.2 -2.7 -0.5 +0.8
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 -1.5 +0.4 -0.3
growth on ypd YPD -0.0 -0.1 -1.6 +0.2 +0.5
growth on ricinoleic acid YNB Methylricinoleic Acid +0.2 -0.1 -0.8 +0.3 -0.6
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.0 -0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.2 +0.4 -0.2 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.1 -0.4 +0.6 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.0 +0.5 -0.2 -0.1
growth on oleic acid YNB Glucose -0.1 +0.3 -0.2 +0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.1 +0.4 -0.3 +0.3
growth on ricinoleic acid YNB Glucose +0.3 +0.1 -0.2 +0.6 -0.3
auxotrophy Dropout Complete -0.0 -0.1 +0.0 +0.7 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.3 +0.6 -0.4 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.0 +0.8 -0.1 +0.1
growth on oleic acid YNB Oleic Acid -0.3 +0.2 +1.0 +0.2 -0.2
auxotrophy YNB Glucose +0.1 +0.1 +0.0 +0.6 +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.2 +1.3 -0.2 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.4 +1.1 -0.1 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 +0.8 +0.7 +0.2
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