Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15473 and 15474 are separated by 87 nucleotides15474 and 15475 are separated by 214 nucleotides15475 and 15476 are separated by 136 nucleotides15476 and 15477 are separated by 323 nucleotides mRNA_7105: 15473 - KOG1890 Phosphoinositide phosphatase SAC1, at 892,209 to 895,612 15473 mRNA_7106: 15474 - K11313 SUPT3H, SPT3 transcription initiation protein SPT3, at 895,700 to 897,201 15474 mRNA_7107: 15475 - KOG2950 Uncharacterized protein involved in protein-protein interaction, contains polyproline-binding GYF domain, at 897,416 to 899,091 15475 mRNA_7108: 15476 - HMMPfam-Arrestin (or S-antigen), C-terminal domain-PF02752,SMART-Arrestin (or S-antigen), C-terminal domain-SM01017,SUPERFAMILY--SSF81296, at 899,228 to 903,559 15476 mRNA_7109: 15477 - HMMPfam-C2 domain-PF00168,ProSiteProfiles-C2 domain profile.-PS50004,SMART-Protein kinase C conserved region 2 (CalB)-SM00239,SUPERFAMILY--SSF49562, at 903,883 to 906,158 15477
Group Condition mRNA_7105 mRNA_7106 mRNA_7107 mRNA_7108 mRNA_7109
15473 15474 15475 15476 15477
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -1.3 -0.4 -0.0 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 +1.0 -2.3 -0.1 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid -0.5 +0.6 -1.9 +0.3 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.5 +0.0 -0.0 -0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 +0.6 -0.9 -0.1 -0.4
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.3 -0.5 -0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.7 -0.6 -0.3 -0.5
growth on oleic acid YNB Glucose +0.1 -0.4 -0.3 -0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 -0.4 -0.0 +0.0
growth on ricinoleic acid YNB Glucose -0.3 +0.4 -0.5 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.6 +0.8 -0.6 +0.0 -0.2
growth on oleic acid YNB Oleic Acid -0.2 +0.1 -0.2 -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.1 -0.6 +0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.0 -0.1 -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.0 -0.0 +0.0 -0.2
auxotrophy YNB Glucose +0.1 +0.5 +0.0 -0.1 -0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +1.3 -0.3 -0.1 -0.4
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.3 +0.7 +0.3 +0.1
auxotrophy Dropout Complete +0.1 +1.1 +0.3 -0.0 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.8 +0.0 +0.8 -0.3 +0.0
growth on ypd YPD +0.5 +1.1 +0.4 +0.1 -0.3
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