Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15477 and 15478 are separated by 370 nucleotides15478 and 15479 are separated by 280 nucleotides15479 and 15480 are separated by 399 nucleotides15480 and 15481 are separated by 211 nucleotides mRNA_7109: 15477 - HMMPfam-C2 domain-PF00168,ProSiteProfiles-C2 domain profile.-PS50004,SMART-Protein kinase C conserved region 2 (CalB)-SM00239,SUPERFAMILY--SSF49562, at 903,883 to 906,158 15477 mRNA_7110: 15478 - HMMPfam-Zinc-binding dehydrogenase-PF00107,HMMPfam-Alcohol dehydrogenase GroES-like domain-PF08240,SMART-Enoylreductase-SM00829,SUPERFAMILY--SSF50129,SUPERFAMILY--SSF51735, at 906,529 to 908,168 15478 mRNA_7111: 15479 - Interpro Subunit 11 of the general transcription factor TFIIH, at 908,449 to 909,688 15479 mRNA_7112: 15480 - Interpro DNA-directed RNA polymerase I subunit RPA34.5 0, at 910,088 to 911,391 15480 mRNA_7113: 15481 - Hypothetical Protein, at 911,603 to 912,181 15481
Group Condition mRNA_7109 mRNA_7110 mRNA_7111 mRNA_7112 mRNA_7113
15477 15478 15479 15480 15481
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.9 -0.7 -0.3 -2.7
growth on ypd YPD -0.3 -0.1 +0.0 +0.1 -1.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.0 -0.3 -0.1 -0.9
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.2 +0.3 +0.1 -0.8
auxotrophy YNB Glucose -0.1 -0.4 +0.3 -0.6 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.4 -0.0 +0.5 -0.3 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.1 +0.3 -0.3 -0.1
auxotrophy Dropout Complete -0.3 +0.1 +0.3 -0.8 +0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.5 -0.0 +0.2 -0.2 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.1 +0.6 -0.4 -0.1
growth on oleic acid YNB Glucose -0.2 -0.2 -0.0 -0.3 +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.2 +0.3 +0.0 -0.5
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.0 +0.1 +0.3 -0.4
growth on ricinoleic acid YNB Glucose -0.1 -0.0 +0.3 +0.1 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 -0.1 +0.4 +0.0 +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.2 +0.5 +0.2 +0.1
growth on oleic acid YNB Oleic Acid +0.1 +0.1 -0.0 -0.0 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 +0.0 +0.2 -0.1 +0.9
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.4 -0.0 +0.5 +0.2 +0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.3 -0.1 +0.2 +1.7
facs on lipid accumulation Sorted High BODIPY Gate -0.4 +0.5 +0.9 +0.1 +0.8
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