Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15479 and 15480 are separated by 399 nucleotides15480 and 15481 are separated by 211 nucleotides15481 and 15482 are separated by 889 nucleotides15482 and 15483 are separated by 637 nucleotides mRNA_7111: 15479 - Interpro Subunit 11 of the general transcription factor TFIIH, at 908,449 to 909,688 15479 mRNA_7112: 15480 - Interpro DNA-directed RNA polymerase I subunit RPA34.5 0, at 910,088 to 911,391 15480 mRNA_7113: 15481 - Hypothetical Protein, at 911,603 to 912,181 15481 mRNA_7114: 15482 - K00383 GSR, gor glutathione reductase (NADPH), at 913,071 to 914,998 15482 mRNA_7115: 15483 - K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH), at 915,636 to 918,075 15483
Group Condition mRNA_7111 mRNA_7112 mRNA_7113 mRNA_7114 mRNA_7115
15479 15480 15481 15482 15483
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 -0.1 -0.9 N.D. -4.7
growth on oleic acid YNB Glucose -0.0 -0.3 +0.5 N.D. -4.9
auxotrophy YNB Glucose 75mM Arginine +0.6 -0.4 -0.1 N.D. -4.7
auxotrophy YNB Glucose +0.3 -0.6 +0.2 N.D. -4.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.3 -0.1 N.D. -4.0
growth on ricinoleic acid YNB Glucose +0.3 +0.1 -0.2 N.D. -4.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.5 +0.2 +0.1 N.D. -4.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.1 +0.9 N.D. -4.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.7 -0.3 -2.7 N.D. +0.5
growth on oleic acid YNB Oleic Acid -0.0 -0.0 +0.5 N.D. -3.5
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.2 +0.3 N.D. -3.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.5 +0.2 +0.7 N.D. -4.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.0 +0.5 N.D. -3.6
facs on lipid accumulation Sorted High BODIPY Gate +0.9 +0.1 +0.8 N.D. -3.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.2 +1.7 N.D. -2.8
growth on ypd YPD +0.0 +0.1 -1.0 N.D. -0.0
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.1 -0.8 N.D. +0.0
auxotrophy Dropout Complete +0.3 -0.8 +0.2 N.D. -0.2
auxotrophy YNB Glucose 75mM Methionine +0.5 -0.3 -0.3 N.D. -0.0
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.0 -0.5 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 -0.4 N.D. +0.2
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