Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 15480 and 15481 are separated by 211 nucleotides15481 and 15482 are separated by 889 nucleotides15482 and 15483 are separated by 637 nucleotides15483 and 15484 are separated by 513 nucleotides mRNA_7112: 15480 - Interpro DNA-directed RNA polymerase I subunit RPA34.5 0, at 910,088 to 911,391 15480 mRNA_7113: 15481 - Hypothetical Protein, at 911,603 to 912,181 15481 mRNA_7114: 15482 - K00383 GSR, gor glutathione reductase (NADPH), at 913,071 to 914,998 15482 mRNA_7115: 15483 - K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH), at 915,636 to 918,075 15483 mRNA_7116: 15484 - K04393 CDC42 cell division control protein 42, at 918,589 to 919,535 15484
Group Condition mRNA_7112 mRNA_7113 mRNA_7114 mRNA_7115 mRNA_7116
15480 15481 15482 15483 15484
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.9 N.D. -4.7 -3.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 N.D. -4.0 -2.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 -2.7 N.D. +0.5 -3.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.7 N.D. -4.4 -1.7
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.1 N.D. -4.7 +0.7
growth on oleic acid YNB Glucose -0.3 +0.5 N.D. -4.9 +0.4
growth on oleic acid YNB Oleic Acid -0.0 +0.5 N.D. -3.5 -1.0
growth on ricinoleic acid YNB Glucose +0.1 -0.2 N.D. -4.3 +0.4
auxotrophy YNB Glucose -0.6 +0.2 N.D. -4.2 +0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.8 N.D. -3.4 -0.8
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 +0.1 N.D. -4.4 +0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.3 N.D. -3.3 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.9 N.D. -4.6 +1.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.5 N.D. -3.6 +1.2
ypd buoyancy 40 hrs Low Buoyancy +0.0 -0.5 N.D. +0.0 -0.7
growth on ypd YPD +0.1 -1.0 N.D. -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.4 N.D. +0.2 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.8 N.D. +0.0 +0.6
auxotrophy Dropout Complete -0.8 +0.2 N.D. -0.2 +1.0
auxotrophy YNB Glucose 75mM Methionine -0.3 -0.3 N.D. -0.0 +1.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +1.7 N.D. -2.8 +2.7
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